Description
Humana Press Inc. Adenovirus Methods And Protocols Volume 1 Adenoviruses Ad Vectors Quantitation And Animal Models by William S. M. Wold
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers._x000D_ _x000D_
TABLE OF CONTENTS_x000D_
Vol. I Topics: Adenoviruses and vectors, quantitation, and animal models_x000D_
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1. Manipulation of Early Region 4_x000D_
Julie Boyer and Gary Ketner_x000D_
2. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants_x000D_
Gary Ketner and Julie Boyer_x000D_
3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus Mutants_x000D_
Peter Groitl and Thomas Dobner_x000D_
4. Construction of Mouse Adenovirus Type 1 Mutants_x000D_
Angela N. Cauthen, Amanda R. Welton, and Katherine R. Spindler_x000D_
5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination System_x000D_
P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti_x000D_
6. Production and Release Testing of Ovine AtadenovirusVectors _x000D_
Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw _x000D_
7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3_x000D_
Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo_x000D_
8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTU_x000D_
Jason Gall, John Schoggins, and Erik Falck-Pedersen_x000D_
9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis_x000D_
T. Subramanian and G. Chinnadurai_x000D_
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10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene _x000D_
Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Seth_x000D_
11. Growth and Purification of Enteric Adenovirus Type 40_x000D_
Vivien Mautner_x000D_
12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses _x000D_
Karoly Toth, Jacqueline F. Spencer, William S.M. Wold_x000D_
13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors_x000D_
Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold_x000D_
14. A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks_x000D_
Maria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Wold_x000D_
15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR_x000D_
C.T. Garnett, Ching-I Pao, Linda R. Gooding_x000D_
16. Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins_x000D_
Graham Bottley, John R. Holt, Nicola J. James and G. Eric Blair_x000D_
17. Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs_x000D_
Amanda R. Welton and Katherine R. Spindler_x000D_
18. Preparation and Titration of CsCl-Banded Adenovirus Stocks _x000D_
Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova, Konstantin_x000D_
Doronin, and William S. M. Wold_x000D_