| This
volume looks at key methodologies that are commonly used across antibody drug
conjugates (ADCs) programs. The chapters in this book cover topics such as
conjugations to endogenous cysteine residues; click chemistry conjugations;
antibody conjugations via glycosyl remodeling; analysis of ADCs by native
mass spectrometry; characterization of ADCs by capillary electrophoresis;
LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC
concentration by ligand-binding assays. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls. Cutting-edge and practical,
Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that
aims to lower the "activation barrier" when undertaking a new
discipline, and provides a "toolbox" for the next generation of ADC
scientists. |