Description
Humana Press Inc. Apoptosis Techniques And Protocols by Andréa C. LeBlanc
In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.
APOPTOSIS TECHNIQUES AND PROTOCOLS, 2ND EDITION
Edited by:
Andrea C. LeBlanc
CONTENTS
Preface to the Series
Preface
Contributors
Molecular Characterization of the Proapoptotic Protein Bax
Yi-Te Hsu and Soraya Smaili
Assessing Cytochrome-c Release from Mitochondria
Douglas W. Ethell and Douglas R. Green
Monitoring Caspases in Neuronal Cell Death
Younes Bounhar, Omar Tounekti, and Andrea C. LeBlanc
HSV Amplicon Vectors in Neuronal Apoptosis Studies
Kathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. Federoff
Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons
Yan Zhang and Andrea C. LeBlanc
Designing Microarray Experiments for Neurobiology
P. Scott Eastman and Jeanne F. Loring
Analysis of Gene Expression by Nylon Membrane cDNA Arrays
H. Michael Tucker and Steven Estus
Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs
Mark D. Berry and Paula C. Ashe
Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons
Barbara Karten, Bronwyn L. MacInnis, Hubert Eng, Yoko Azumaya, Grace Martin, Karen Lund, Russell C. Watts, Jean E. Vance, Dennis E. Vance, and Robert B. Campenot
Detection and Analysis of Synaptosis
Greg M. Cole and Karen Gylys
In Situ Detection of Apoptotic Neurons
Kevin A. Roth
Apoptotic and Oxidative Indicators in Alzheimer's Disease
Arun K. Raina, Lawrence M. Sayre, Craig S. Atwood, Catherine A. Rottkamp, Ayala Hochman, Xiongwei Zhu, Mark E. Obrenovich, Shun Shimohama, Akihiko Nunomura, Atsushi Takeda, George Perry, and Mark A. Smith
Index