Description
Humana Press Atomic Force Microscopy in Biomedical Research Methods and Protocols 1st Editon 2011 Hardbound by Pier Carlo Braga, Davide Ricci
With its ability to explore the surface of the sample by means of a local scanning probe and its use of dedicated software allows to be visualize results, atomic force microscopy (AFM) has revolutionized the study of the smallest aspects of life. Atomic Force Microscopy in Biomedical Research: Methods and Protocols proves that this technology is no longer simply just another form of microscopy but has given rise to a completely new way of using microscopy that fulfils the dreams of all microscopists: being able to touch, move, and interact with the sample while it is being examined, thus making it possible to discover not only morphological but also chemical and physical structural information. Covering such topics as molecule imaging, nanoscale surface analysis and cellular imaging, force-spectroscopy, investigating drug action, and AFM as a nanotool, this volume features the most up-to-date techniques currently in use. Written in the Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Atomic Force Microscopy in Biomedical Research: Methods and Protocols brings together different types of applications in order to provide examples from diverse fields in the hope of inspiring researchers to apply their ingenuity in their own specialization and add significant originality to their varying studies. How the Atomic Force Microscope Works.- Measurement Methods in Atomic Force Microscopy.- Recognizing and Avoiding Artifacts in Atomic Force Microscopy Imaging.- Imaging the Spatial Orientation of Subunits within Membrane Receptors by Atomic Force Microscopy.- High Resolution Imaging of Immunoglobulin G (IgG) Antibodies and Other Biomolecules Using Amplitude Modulation Atomic Force Microscopy in Air.- Atomic Force Microscopy of Ex vivo Amyloid Fibrils.- Studying Collagen Self-Assembly by Time-Lapse High-Resolution Atomic Force Microscopy.- Atomic Force Microscopy Imaging of Human Metaphase Chromosomes in Liquid.- Atomic Force Microscopy of Proteasome Assemblies.- Atomic Force Microscopy of Isolated Mitochondria.- Imaging Native Membrane Proteins Using the Atomic Force Microscope.- Atomic Force Microscopy Investigation of Viruses.- Determination of the Kinetic On and -Off Rate of Single Virus-Cell Interactions.- Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites.- Normal and Pathological Erythrocytes Studied by Atomic Force Microscopy.- The Growth Cones of Living Neurons Probed by the Atomic Force Microscope.- Highlights on Ultrastructural Pathology of Human Sperm.- High-speed Atomic Force Microscopy and Biomolecular Processes.- Atomic Force Microscopy in Mechanobiology: Measuring Micro-Elastic Heterogeneity of Living Cells.- Force-Clamp Measurements of Receptor-Ligand Interactions.- Measuring Cell Adhesion Forces: Theory and Principles.- Nanoscale Investigation on E. coli Adhesion to Modified Silicone Surfaces.- Imaging Bacterial Shape, Surface, and Appendages Before and After Treatment with Antibiotics.- Thymol-Induced Alterations in Candida albicans Imaged by Atomic Force Microscopy.- Atomic Force Microscope-Enabled Studies of Integrin-Extracellular Matrix Interactions in Vascular Smooth Muscle and Endothelial Cells.- Atomic Force Microscopy Studies on Circular DNA Structural Changes by Vincristine and Aspirin.- Combined Atomic Force Microscopy and Fluorescence Microscopy.- Chemical Modifications of Atomic Force Microscopy Tips.- Atomic Force Microscopy as Nanorobot.