Description
CBS PUBLICATION Biochemical Techniques Theory And Practice by Robyt J F
Biochemical Techniques presents the fundamental theory and practice used in biochemistry laboratory courses and in biochemical research. Chapter I covers the making of measurements and error analysis, the presentation of data in tables and graphs, and the writing of reports. Chapter 2 presents the preparation and properties of solutions and includes pH and buffers. For some students, the contents of Chapters I and 2 may already have been covered in other chemistry courses. Chapter 3 proceeds with qualitative and quantitative techniques including UV and visible spectrophotometry, fluorimetry, and infrared and nuclear magnetic resonance spectroscopy. Chapter 4 covers chromatographic methods of analysis and is divided into two parts. Part I discusses the general aspects of the many types of chromatography and Part II covers specific applications for separating carbohydrates, amino acids, proteins, nucleic acids, and lipids. Chapter 5 presents electrophoretic methods of separation and places emphasis on zone electrophoresis, especially polyacrylamide- and agarose-gel electrophoresis. Chapter 6 discusses the nature and properties of radioisotopes and techniques for quantitatively measuring radioactivity by gas-ionization, liquid and solid scintillation spectrometry, and autoradiography. Specific methods of radioisotope labeling of biochemical compounds and applications of their use in tracer experiments are presented. Chapter 6 concludes with a discussion of the points that should be considered in designing an experiment using radioisotopes.