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Biochemical Techniques Theory And Practice
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Biochemical Techniques presents the fundamental theory and practice used in biochemistry laboratory courses and in biochemical research. Chapter I covers the making of measurements and error analysis, the presentation of data in tables and graphs, and the writing of reports. Chapter 2 presents the preparation and properties of solutions and includes pH and buffers. For some students, the contents of Chapters I and 2 may already have been covered in other chemistry courses. Chapter 3 proceeds with qualitative and quantitative techniques including UV and visible spectrophotometry, fluorimetry, and infrared and nuclear magnetic resonance spectroscopy. Chapter 4 covers chromatographic methods of analysis and is divided into two parts. Part I discusses the general aspects of the many types of chromatography and Part II covers specific applications for separating carbohydrates, amino acids, proteins, nucleic acids, and lipids. Chapter 5 presents electrophoretic methods of separation and places emphasis on zone electrophoresis, especially polyacrylamide- and agarose-gel electrophoresis. Chapter 6 discusses the nature and properties of radioisotopes and techniques for quantitatively measuring radioactivity by gas-ionization, liquid and solid scintillation spectrometry, and autoradiography. Specific methods of radioisotope labeling of biochemical compounds and applications of their use in tracer experiments are presented. Chapter 6 concludes with a discussion of the points that should be considered in designing an experiment using radioisotopes.

More Details About Biochemical Techniques Theory And Practice
General Information
Author(s)Robyt J.F.
PublisherCBS Publisher
Publish YearJanuary 2015
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