Description
Humana Press Cancer Cell Signaling Methods and Protocols 1st Editon 2010 Softbound by David M. Terrian
Cells respond to environmental cues through a complex and dynamic network of signaling pathways that normally maintain a critical balance between cellular proliferation, differentiation, senescence, and death. One current research challenge is to identify those aberrations in signal transd- tion that directly contribute to a loss of this division-limited equilibrium and the progression to malignant transformation. The study of cell-signaling m- ecules in this context is a central component of cancer research. From the knowledge of such targets, investigators have been able to productively advance many insightful hypotheses about how a particular cancer cell may misinterpret, or respond inappropriately to, growth regulatory cues in their environment. Despite these key insights, the rapidly evolving nature of cell signaling research in cancer has necessitated a continuous revision of these theoretical constructs and the updating of methods used in their study. One contemporary example of the evolution of this field is provided by an analysis of the Human Genome Project data, which reveal a previously unsuspected diversity in the multigene families encoding for most signaling pathway int- mediates. In assessing the usefulness of a particular methodological approach, therefore, we will need to keep in mind that there is a premium on those p- tocols that can be easily adapted for the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together several such methods in cell signaling research that are scientifically grounded within the cancer biology field. Manipulation and Detection of Survival Signals.- Functional Analysis of the Antimitogenic Activity of Tumor Suppressors.- Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination.- Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells.- The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress.- Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression.- Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells.- TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s).- Manipulation and Edtection of Oncogenic Signals.- Kinetworks™ Protein Kinase Multiblot Analysis.- Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers.- Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay.- Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay.- Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells.- Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD).- Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model.- Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer.- Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes.- Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes.- Protein Interactions.- Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology.- Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay.- Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions.- Genomic Rearrangements.- Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing.- Clonality Analysis by T-Cell Receptor ? PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL).