Description
Humana Cell Cycle Control and Dysregulation Protocols by Antonio Giordano, Gaetano Romano
Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Because there are already many manuals and protocols available, along with commercial kits and reagents, a variety of the more common techniques have not been included in our book. The protocols described, based on rather sophisticated techniques for in vivo and in vitro studies, consist of molecular biology, biochemistry, and various types of immunoassays. Indeed, the authors have successfully accomplished an arduous task by presenting several topics in the simplest possible manner. We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book._x000D_ Table of contents : - _x000D_
Part I. Analysis of Cyclins and Cyclin-Dependent Kinases_x000D_
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The Biology of Cyclins and Cyclin-Dependent Protein Kinases: An Introduction_x000D_
Lucio Miele_x000D_
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In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy_x000D_
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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In Situ Immunofluorescence Analysis: Analyzing RNA Synthesis by 5-Bromouridine-5'-Triphosphate Labeling_x000D_
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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Immunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro System_x000D_
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins_x000D_
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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Chromatin Immunoprecipitation_x000D_
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Electrophoretic Mobility Shift Assay_x000D_
AmjadJaved, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I Digestion_x000D_
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase Chain Reaction_x000D_
Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein_x000D_
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Assays for Cyclin-Dependent Kinase Inhibitors_x000D_
Adrian M. Senderowicz. Protein Degradation Via the Proteosome, Henry Hoff, Hong Zhang, and Christian Sell_x000D_
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Part II. Analysis of the Factors Involved in Cell Cycle Deregulation_x000D_
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The Transformed Phenotype_x000D_
Henry Hoff, Barbara Belletti, Hong Zhang, and Christian Sell_x000D_
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A Morphologic Approach to Detect Apoptosis Based on Electron Microscopy_x000D_
Martyn K. White and Caterina Cinti_x000D_
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Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation_x000D_
Carmela Trimarchi, Dario La Sala, Alessandra Zamparelli, and Caterina Cinti_x000D_
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Part III. Cellular Response to Deoxyribonucleic Acid Damage_x000D_
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Induction of Deoxyribonucleic Acid Damage by Alkylating Agents_x000D_
Salvatore Cortellino, David P. Turner, Domenico Albino, and Alfonso Bellacosa_x000D_
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Induction of Deoxyribonucleic Acid Damage by g Irradiation_x000D_
Salvatore Cortellino, David P. Turner and Alfonso_x000D_