Description
Humana Cilia Methods and Protocols by Peter Satir, Soren Tvorup Christensen
This volume is the result of an explosion of_x000D_molecular-based research on Cilia, which began with the discovery of the_x000D_universality of intraflagellar transport (IFT) and ciliary genomics/proteomics._x000D_The chapters in this book cover topics such as: high resolution imaging and_x000D_functional characterization of sensory and primary cilia in mammalian cells and_x000D_zebrafish, methods to study ciliary-mediated chemoresponse in Paramecium,_x000D_and methods to study centrosomes and cilia in C. elegans and Drosophila._x000D_Written in the highly successful Methods in Molecular Biology series format,_x000D_chapters include introduction to their respective topics, lists of the_x000D_necessary materials and reagents, step-by-step, readily reproducible laboratory_x000D_protocols, and tips on troubleshooting and avoiding known pitfalls._x000D__x000D_Practical and cutting-edge, Cilia:_x000D_Methods and Protocols is broad and covers motile, sensory, and primary_x000D_cells. It is a valuable resource to anyone interested in entering the field of_x000D_ciliary biology using model organisms, including flagellate algae, ciliates,_x000D_planaria, nematodes, insects, zebrafish, and mammalian cells._x000D_ Table of contents : - _x000D_
1. Methods for Studying Ciliary Import Mechanisms_x000D_
Daisuke Takao and Kristen J._x000D_
Verhey_x000D_
_x000D_
2. Targeting of ASH Domain-Containing Proteins to_x000D_
the Centrosome_x000D_
Patricia Verdier, Stine K._x000D_
Morthorst, and Lotte B. Pedersen_x000D_
_x000D_
3. Morphological and Functional Characterization_x000D_
of the Ciliary Pocket by Electron and Fluorescence Microscopy_x000D_
Rania Ghossoub, Louise Lindbaek, Anahi Molla-Herman, Alain Schmitt, Soren Tvorup Christensen, and_x000D_
Alexandre Benmerah_x000D_
_x000D_
4. Methods to Study Interactions between Ciliogenesis_x000D_
and Autophagy_x000D_
Birgit H. Satir and Olatz_x000D_
Pampliega_x000D_
_x000D_
5. Recombinant Reconstitution and Purification of_x000D_
the IFT B Core Complex from Chlamydomonas_x000D_
reinhardtii_x000D_
Michael Taschner and Esben_x000D_
Lorentzen_x000D_
_x000D_
6. Methods for Studying Movement of Molecules_x000D_
within Cilia_x000D_
Karl F. Lechtreck_x000D_
_x000D_
7. A FRAP-Based Method for Monitoring Molecular Transport_x000D_
in Ciliary Photoreceptor Cells In Vivo_x000D_
Kirsten A. Wunderlich and_x000D_
Uwe Wolfrum_x000D_
_x000D_
8. Kymographic _x000D_
Analysis of Transport in an Individual Neuronal Sensory Cilium in Caenorhabditis elegans_x000D_
Robert O'Hagan and Maureen_x000D_
M. Barr_x000D_
_x000D_
9. Visualization and Manipulation of Cilia and Intraciliary_x000D_
Calcium in the Zebrafish Left-Right Organizer_x000D_
Shiaulou Yuan and Martina_x000D_
Brueckner_x000D_
_x000D_
10. Methods for Studying Ciliary-Mediated Chemoresponse_x000D_
in Paramecium_x000D_
M.S. Valentine and J.L._x000D_
Van Houten_x000D_
_x000D_
11. STED_x000D_
and STORM Superresolution Imaging of Primary Cilia_x000D_
T. Tony Yang, Weng Man Chong,_x000D_
and Jung-Chi Liao_x000D_
_x000D_
12. CLEM Methods for Studying Primary Cilia_x000D_
Frank P. Macaluso, Geoffrey S._x000D_
Perumal, Johan Kolstrup, and Peter Satir_x000D_
_x000D_
13. Methods for Visualization of Neuronal Cilia_x000D_
Tamara Caspary, Daniela_x000D_
Marazziti, and Nicolas F. Berbari_x000D_
_x000D_
14. Methods to Study Centrosomes and Cilia in Drosophila_x000D_
Swadhin Chandra Jana, Susana Mendonca, Sascha Werner, and Monica Betterncourt-Dias_x000D_
_x000D_
15. Analysis of Axonemal Assembly during Ciliary Regeneration_x000D_
in Chlamydomonas_x000D_
Emily L. Hunter, Winfield_x000D_
S. Sale, and Lea M. Alford_x000D_
_x000D_
16. Planaria as a_x000D_
Model System for the Analysis of Ciliary Assembly and Motility_x000D_
Stephen M. King and Ramila S._x000D_
Patel-King_x000D_