Description
Humana Press Inc. Clinical And Forensic Applications Of Capillary Electrophoresis by John R. Petersen, Amin A. Mohammad
John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.
Section I. Introduction
Capillary Electrophoresis: General Overview and Applications in the Clinical Laboratory
Manjiri Lele, Subodh M. Lele, John R. Petersen, and Amin A. Mohammad
Basic Principles and Modes of Capillary Electrophoresis
Harry Whatley
Section II. Protein Electrophoresis
Capillary Coatings for Protein Analysis
Kannan Srinivasan, Chris Pohl, and Nebojsa Avdalovic
Clinical Serum Protein Capillary Zone Electrophoresis
Carl R. Jolliff
Serum and Urine Paraprotein Capillary Electrophoresis
Carl R. Jolliff
Cerebrospinal Fluid Protein Electrophoresis
John R. Petersen and Amin A. Mohammad
Lipoprotein Analysis
Rainer Lehmann
Clinical Analysis of Structural Hemoglobin Variants and Hb A1c by Capillary Isoelectric Focusing
James M. Hempe, Alfonso Vargas, and Randall D. Craver
Section III. Metabolic Diseases
Amino Acid Analysis, Mary Kathryn Linde. Organic Acids
Kern L. Nuttall and Norberto A. Guzman
Steroids, Cecilla Youh
Amin A. Mohammad, and John R. Petersen
Section IV. Immunoassay
Capillary Electrophoresis Based Immunoassay
Bode Adesoji, Amin A. Mohammad, and John R. Petersen
Section V. Molecular Diagnostics
Quantitation of Viral Load
Jill M. Kolesar
The Application of Capillary Electrophoresis in the Analysis of PCR Products used in Forensic DNA Typing
Bruce R. McCord and John M. Butler
Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry
Samir Cherkaoui
Section VI. Mass Spectrometry, Therapeutic Drug Monitoring, and Toxicology
Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins
Stephen Naylor and Andy J. Tomlinson
Serum Drug Monitoring by Capillary Electrophoresis
Zack K. Shihabi
Applications of Capillary Zone Electrophoresis in the Analysis of Metal Ions of Clinical Significance
Lokinendi V. Rao, John R. Petersen, Amin A. Mohammad, and Anthony O. Okorodudu
Clinical and Forensic Drug Toxicology: Analysis of Illicit and Abused Drugs in Urine by Capillary Electrophoresis
Wolfgang Thormann and Jitka Caslavska
Screening Biological Specimens for Drugs of Forensic Significance
John C. Hudson, Murray J. Malcolm, and Mauro Golin