Description
Springer Crop Breeding Genetic Improvement Methods 2021 Edition by Tripodi P.
This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls._x000D__x000D__x000D__x000D__x000D__x000D__x000D__x000D_Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methods aims to ensure successful results in the further study of this vital field._x000D_ Table of contents :- 1. Methods of Development of Biparental Mapping Populations in Horticultural CropsPasquale Tripodi2. Multiparental Population in Crops: Methods of Development and Dissection of Genetic TraitsIsidore Diouf and Laura Pascual3. Using Metabolomics to Assist Plant BreedingSaleh Alseekh and Alisdair R. Fernie4. High Throughput DNA Isolation in Vegetable Crops for Genomics ApplicationsPasquale Tripodi and Giovanna Festa5. High Resolution Melting Analysis as a Tool for Plant Species Authentication Liliana Grazina, Joana Costa, Joana S. Amaral, and Isabel Mafra6. Specific-locus Amplified Fragment Sequencing (SLAF-seq) as High-Throughput SNP Genotyping MethodsZhangsheng Zhu, Binmei Sun, and Jianjun Lei7. Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato GenomeFernando J. Yuste-Lisbona, Jose M. Jime nez-Go mez, Carmen Capel, and Rafael Lozano8. Association Mapping in PlantsPawan L. Kulwal and Ravinder Singh9. Practical Workflow from High Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs)Contaldi Felice, Cappetta Elisa, and Esposito Salvatore10. Guidelines for Setting up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis Teresa Rosa Galise, Salvatore Esposito, and Nunzio D'Agostino 11. RNA Interference (RNAi) in Tomato Crop ReseserchPasquale Termolino12. Protoplast Based Method for Genome Editing in Tetraploid PotatoAlessandro Nicolia, Ann-Sofie Falt, Per Hofvander, and Mariette Andersson13. The Double Layer Method to the Genesis of Androgenic Plants in Anemone coronariaAndrea Copetta and Marina Laura14. Ploidy Modification for Plant Breeding Using in vitro Organogenesis: a Case in Eggplant Edgar Garcia-Fortea, Ana Garcia-Perez, Esther Gimeno-Paez, Marina Martinez-Lopez, Santiago Vilanova, Pietro Gramazio, Jaime Prohens and Mariola Plazas15. Assembly of TALEN and mTALE-Act for Plant Genome EngineeringAimee A. Malzahn and Yiping Qi16. Genome Editing to Achieve the Crop Ideotype in TomatoTomas Cermak, Karla Gasparini, Zoltan Kevei, and Agustin Zsoegoen17. Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral ImagingGernot Bodner, Mouhannad Alsalem, and Alireza Nakhforoosh18. Light Drones for Basic In-field Phenotyping and Precision Farming Applications: RGB Tools Based on Image AnalysisFederico Pallottino, Simone Figorilli, Cristina Cecchini, and Corrado Costa