Description
SPRINGER Cryopreservation And Freeze Drying Protocols 4Ed (Pb 2021) by WOLKERS W.F.
This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.The chapter "Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. Part I: Fundamental Aspects of Cryopreservation and Freeze-Drying1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and TissuesWillem F. Wolkers and Harriette Oldenhof2. Principles of Ice-Free Cryopreservation by VitrificationGregory M. Fahy and Brian Wowk3. The Principles of Freeze-Drying and Application of Analytical TechnologiesKevin R. Ward and Paul Matejtschuk4. Mathematical Modeling and Optimization of Cryopreservation in Single CellsJames D. Benson5. Mathematical Modeling of Protectant Transport in TissuesRoss M. Warner and Adam Z. HigginsPart II: Technologies and Methods to Study Freezing and Drying6. Freezing Technology: Control of Freezing, Thawing, and Ice NucleationPeter Kilbride and Julie Meneghel7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of BiologicsShangping Wang, Susan Trammell, and Gloria D. Elliott8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation KineticsJens O.M. Karlsson9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice RecrystallizationAnna A. Ampaw, August Sibthorpe, and Robert N. Ben10. DSC Analysis of Thermophysical Properties for Biomaterials and FormulationsWendell Q. Sun11. Osmometric Measurements of Cryoprotective Agent Permeation into TissuesKezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation ProcessesAriadna Corral, Alberto Olmo, and Ramon Risco13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological ResearchWillem F. Wolkers and Harriette Oldenhof14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature MeasurementsGuanglin Yu, Rui Li, and Allison HubelPart III: Cryopreservation and Freeze-Drying Protocols15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine SpermHarriette Oldenhof, Willem F. Wolkers, and Harald Sieme16. Cryopreservation of Avian SemenHenri Woelders17. Cryopreservation of Mouse Sperm for Genome BankingYuksel Agca and Cansu Agca18. Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval StagesEstefania Paredes, Pablo Heres, Catarina Anjos, and Elsa Cabrita19. Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling AgentMengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, and Vladimir Isachenko20. Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic StorageVictoria Keros and Barry J. Fuller21. Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid NitrogenTamas Somfai and Kazuhiro Kikuchi22. Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical ResearchYuksel Agca and Cansu Agca23. Cryopreservation and Thawing of Human Ovarian Cortex Tissue SlicesJana Liebenthron and Markus Montag24. Vitrification: A Simple and Successful Method for Cryostorage of Human BlastocystsJuergen Liebermann25. Vitrification of Equine In Vivo-Derived Embryos, after Blastocoel AspirationCarolina Herrera26. Frozen Blood ReservesJohan W. Lagerberg27. Isolation, Cryopreservation, and Characterization of iPSC-Derived MegakaryocytesDenys Pogozhykh, Rainer Blasczyk, and Constanca Figueiredo28. Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem CellsMelany Lopez and Ali Eroglu29. Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem CellsJuliette Seremak and Ali Eroglu30. Protocol for Cryopreservation of Endothelial MonolayersLeah A. Marquez-Curtis, Nasim Eskandari, Locksley E. McGann, and Janet A.W. Elliott31. Vitrification of Heart Valve TissuesKelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell32. Cryopreservation of AlgaeEstefania Paredes, Angela Ward, Ian Probert, Lena Gouhier, and Christine N. Campbell33. Cryopreservation of Fern Spores and PollenAnna Nebot, Victoria J. Philpott, Anna Pajdo, and Daniel Ballesteros34. Cryopreservation of Plant Cell Lines Using Alginate EncapsulationHeinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack35. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3Angelika Senula and Manuela Nagel36. Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded SpeciesDaniel Ballesteros, Natalia Fanega-Sleziak, and Rachael Davies37. Freeze-Drying of ProteinsBaolin Liu and Xinli Zhou38. Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical PropertiesFernanda Fonseca, Amelie Girardeau, and Stephanie Passot39. Preservation of Mammalian Sperm by Freeze-DryingLevent Keskintepe and Ali Eroglu40. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf AvailabilityWillem F. Wolkers and Andres Hilfiker