Description
Humana Diabetes Mellitus Methods And Protocols by Sabire OEzcan
Diabetes mellitus is the collective name for a group of diseases associated with hyperglycemia (high levels of blood glucose) caused by defects in insulin p- duction, insulin action, or both. About 6. 2% of the US population (17 million people) have diabetes mellitus. It is the leading cause of kidney failure, bli- ness, and amputations. It is also a major risk factor for heart diseases, stroke, and birth defects. Diabetes Mellitus: Methods and Protocols provides a state-of-the-art account of the experimental methodology for studying the molecular defects leading to diabetes mellitus, both at the molecular and biochemical levels. The chapters cover a wide range of topics written by experts in their respective fields and are organized in two sections: Insulin Production and Insulin Action. The detailed experimental protocols presented, including the notes of interest, provide a very useful tool for basic researchers and clinicians for investigating and treating this disease. Each chapter starts with an introduction to a specific technique and explains its application in the field of diabetes research. Following the list of materials, a detailed description of the technique is presented in the methods section in a way that enables the successful execution of the protocol. The "Notes" section at the end discusses the pitfalls of the technique and alternative approaches. I am grateful to the numerous scientists who have contributed to this volume by writing both highly detailed and understandable chapters. Part I. Insulin ProductionIsolation of Islets of Langerhans from Rodent PancreasColleen B. Kelly, Libby A. Blair, John A. Corbett, and Anna L. ScarimPurification of Rat Pancreatic b-Cells by Fluorescence-Activated Cell SortingGeert Stange, Mark Van De Casteele, and Harry HeimbergAssessment of Insulin Secretion in the MouseMarcela Brissova, Wendell E. Nicholson, Masakazu Shiota, and Alvin C. PowersDetection of Insulin Production by ImmunohistochemistrySusan C. Campbell and Wendy M. MacfarlaneQuantification of the Level of Insulin Gene ExpressionSusan C. Campbell and Wendy M. MacfarlaneChromatin Immunoprecipitation Using Isolated Islets of LangerhansMarcelina Parrizas, Sylvia F. Boj, Reini F. Luco, Miguel Angel Maestro, and Jorge FerrerAdenoviral Gene Transfer into b-Cell LinesAmber L. Mosley and Sabire OEzcanUtilization of NOD Mice in the Study of Type 1 DiabetesDennis G. Karounos and Susan E. GoesPart II. Insulin ActionIntroduction of DNA into 3T3-L1 Adipocytes by ElectroporationShuichi Okada, Masatomo Mori, and Jeffrey E. PessinAnalysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 AdipocytesJaganathan Lakshmanan, Jeffrey S. Elmendorf, and Sabire OEzcanFractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 AdipocytesJeffrey S. ElmendorfVisualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet AssayJohn B. Knight and Ann Louise OlsonAssaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor SubstratesEileen L. Whiteman and Morris J. BirnbaumMeasuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase ActivityLi-Ping Wang and Scott A. SummersAssaying AKT/ Protein Kinase B ActivityKyle L. Hoehn and Scott A.SummersMeasurements of Cellular Phosphoinositide Levels in 3T3-L1 AdipocytesDaryll B. DeWaldMeasurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 AdipocytesMatthew J. BradyMeasurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal MuscleJoseph T. Brozinick, Jr.Single Embryo Measurement of Basal- and Insulin-Stimulated Glucose UptakeMaggie M-Y. Chi and Kelle H. MoleyImmunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose TransportersMary O. Carayannopoulos and Kelle H. MoleyIndex