Directed Evolution Library Creation Methods And Protocols (Methods In Molecular Biology Volume 231) by ARNOLD FRANCES H ET AL, HUMANA PRESS

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HUMANA PRESS Directed Evolution Library Creation Methods And Protocols (Methods In Molecular Biology Volume 231) by ARNOLD FRANCES H ET AL

Biological systems are very special substrates for engineering-uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme engineering, directed evolution has matured to the point where it now represents the centerpiece of industrial biocatalyst development and is being practiced by thousands of academic and industrial scientists in com- nies and universities around the world. The appeal of directed evolution is easy to understand: it is conceptually straightforward, it can be practiced without any special instrumentation and, most important, it frequently yields useful solutions, many of which are totally unanticipated. Directed evolution has r- dered protein engineering readily accessible to a broad audience of scientists and engineers who wish to tailor a myriad of protein properties, including th- mal and solvent stability, enzyme selectivity, specific activity, protease s- ceptibility, allosteric control of protein function, ligand binding, transcriptional activation, and solubility. Furthermore, the range of applications has expanded to the engineering of more complex functions such as those performed by m- tiple proteins acting in concert (in biosynthetic pathways) or as part of mac- molecular complexes and biological networks._x000D_ _x000D_ Part I. Mutagenesis and Recombination Methods_x000D_ _x000D_ Generating Mutant Libraries Using Error-Prone PCR_x000D_ Patrick C. Cirino, Kimberly M. Mayer, and Daisuke Umeno_x000D_ _x000D_ Preparing Libraries in Escherichia coli_x000D_ Alexander V. Tobias_x000D_ _x000D_ Preparing Libraries in Saccharomyces cerevisiae_x000D_ Thomas Bulter and Miguel Alcalde_x000D_ _x000D_ Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP)_x000D_ Kentaro Miyazaki_x000D_ _x000D_ Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis_x000D_ Paul H. Bessette, Marco A. Mena, Annalee W. Nguyen, and Patrick S. Daugherty_x000D_ _x000D_ Production of Randomly Mutated Plasmid Libraries Using Mutator Strains_x000D_ Annalee W. Nguyen and Patrick S. Daugherty_x000D_ _x000D_ Evolution of Microorganisms Using Mutator Plasmids_x000D_ Olga Selifonova and Volker Schellenberger_x000D_ _x000D_ Random Insertion and Deletion Mutagenesis_x000D_ Hiroshi Murakami, Takahiro Hohsaka, and Masahiko Sisido_x000D_ _x000D_ Random Oligonucleotide Mutagenesis_x000D_ Jessica L. Sneeden and Lawrence A. Loeb_x000D_ _x000D_ Saturation Mutagenesis_x000D_ Radu Georgescu, Geethani Bandara, and Lianhong Sun_x000D_ _x000D_ DNA Shuffling_x000D_ John M. Joern_x000D_ _x000D_ Family Shuffling with Single-Stranded DNA_x000D_ Wenjuan Zha, Tongbo Zhu, and Huimin Zhao_x000D_ _x000D_ In Vitro DNA Recombination by Random Priming_x000D_ Olga Esteban, Ryan D. Woodyer, and Huimin Zhao_x000D_ _x000D_ Staggered Extension Process (StEP) In Vitro Recombination_x000D_ Anna Marie Aguinaldo and Frances H. Arnold_x000D_ _x000D_ RACHITT: Gene Family Shuffling by Random Chimeragenesis on Transient Templates_x000D_ Wayne M. Coco_x000D_ _x000D_ The Creation of ITCHY Hybrid Protein Libraries_x000D_ Marc Ostermeier and Stefan Lutz_x000D_ _x000D_ Preparation of SCRATCHY Hybrid Protein Libraries: Size- and In-Frame Selection of Nucleic Acid Sequences_x000D_ Stefan Lutz and Marc Ostermeier_x000D_ _x000D_ Sequence Homology-Independent Protein Recombination (SHIPREC)_x000D_ Andrew K. Udit, Jonathan J. Silberg,and Volker Sieber_x000D_ _x000D_ Producing Chimeric Genes by CLERY: In Vitro and In Vivo Recombination_x000D_ Valerie Abecassis, Denis Pompon, and Gilles Truan_x000D_ _x000D_ Part II. Analysis of Library Diversity_x000D_ _x000D_ Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization_x000D_ Peter Meinhold, John M. Joern, and Jonathan J. Silberg_x000D_ _x000D_ Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays: Experimental Design of DNA Arrays_x000D_ Valerie Abecassis, Gilles Truan, Loic Jaffrelo, and Denis Pompon_x000D_ _x000D_ Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays: Bioinformatic Treatment of Data_x000D_ Denis Pompon, Gilles Truan, and Valerie Abecassis_x000D_ _x000D_ Index_x000D_