Description
Springer Dna Vaccines Methods And Protocols 2021 Edition by Sousa A.M.A.D.
This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls._x000D__x000D__x000D__x000D__x000D__x000D__x000D__x000D_Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field._x000D_ Table of contents :- Part I: Vector design For vaccination1. A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary ApplicationYanlong Jiang, Guilian Yang, and Chunfeng Wang2. Vaccination with Messenger RNA - a Promising Alternative to DNA VaccinationIstvan Tombacz, Drew Weissman, and Norbert Pardi3. Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization EfficiencyKenneth LundstromPart II: DNA Vaccine Adjuvants and Immunostimulatory Response 4. CpG Oligonucleotides as Vaccine AdjuvantsNeslihan Kayraklioglu, Begum Horuluoglu, and Dennis M. Klinman5. Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and FormulationAilar Sabbaghi and Amir Ghaemi6. Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate ModelXintao Hu, Barbara K. Felber, and Antonio ValentinPart III Biotechnological Processes to Obtain DNA Vaccines7. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental DesignLuis A. Passarinha8. Primary Purification of Plasmid DNA Using Differential Isopropanol PrecipitationAlexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, and Duarte Miguel F. Prazeres9. Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic MonolithsUrh Cernigoj and Ales Strancar10. Purification of Plasmid DNA by Multimodal ChromatographyA. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, and Ana M. Azevedo11. Minicircle DNA Vaccine Purification and E7 Antigen Expression AssessmentAna M. Almeida, Dalinda Eusebio, Joao A. Queiroz, Fani Sousa, and Angela SousaPart IV: DNA Vaccine Delivery 12. Tumor-Specific CD8+ T Cell Responses Induced by DNA VaccinationPablo Caceres-Morgado and Alvaro Lladser13. Therapeutic DNA Vaccine Against HPV16-Associated CancerMeihua Yu and Janin Chandra14. Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery ApplicationsLucimara Gaziola de la Torre, Amanda da Costa e Silva de Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Es, and Gabriel Perli15. Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines FieldDiana Costa, Angela Sousa, Ruben Faria, Ana Raquel Neves, and Joao A. Queiroz16. Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update ProtocolsNina D. Coelho-Rocha, Fernanda A. L. Barroso, Laisa M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond, and Pamela Mancha-AgrestiPart V: DNA Vaccine Transfer to Clinical Trials17. Ethics of DNA Vaccines Transfer for Clinical ResearchAna Cristina Ramalhinho and Miguel Castelo-Branco18. Preparation of an Academic Clinical TrialPreparation of an academic clinical trialAna Cristina Ramalhinho and Miguel Castelo-Branco