Description
Humana Embryonic Stem Cell Therapy For Osteo-Degenerative Diseases Methods And Protocols 2010 Edition by Nicole I. Nieden
Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity as opposed to the limits of adult stem cells; therefore ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo i.e. mouse and human in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice be it osteoblasts osteoclasts or chondrocytes and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology (TM) series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and expert tips on troubleshooting and avoiding known pitfalls.Dependable and cutting-edge Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy. Table of contents : 1. Embryonic Stem Cells for Osteo-Degenerative DiseasesNicole I. zur Nieden2. Methods for Culturing Mouse and Human Embryonic Stem CellsSabrina Lin and Prue Talbot3. Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem CellsLudovic Vallier4. Functional Assays for Human Embryonic Stem Cell PluripotencyMichael D. O'Connor Melanie D. Kardel and Connie J. Eaves5. Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem CellsHelen Spencer Maria Keramari and Christopher M. Ward6. Generation of Human Embryonic Stem Cells Carrying Lineage Specific ReportersParinya Noisa Alai Urrutikoetxea-Uriguen and Wei Cui7. Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their DerivativesStephanie N. Rushing Anthony W. Herren Deborah K Lieu and Ronald A. Li8. Large-Scale Expansion of Mouse Embryonic Stem Cells on MicrocarriersAna M. Fernandes Maria Margarida Diogo Claudia Lobato da Silva and Joaquim M.S. Cabral9. Embryoid Body Formation: Recent Advances in Automated Bioreactor TechnologySusanne Trettner Alexander Seeliger and Nicole I. zur Nieden10. Methods for Embryoid Body Formation: The Microwell ApproachDawn P. Spelke Daniel Ortmann Ali Khademhosseini Lino Ferreira and Jeffrey M. Karp11. Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An OverviewPeiman Hematti12. Derivation of Mesenchymal Stem Cells from Human Embryonic Stem CellsAndre Choo and Sai Kiang Lim13. Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the "Raclure" MethodEmmanuel N. Olivier and Eric E. Bouhassira14. Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and ChondrocytesBeatrice Kuske Vuk Savkovic and Nicole I. zur Nieden15. Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide ScaffoldsNuria Mari-Buye and Carlos E. Semino16. Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem CellsMotokazu Tsuneto Toshiyuki Yamane and Shin-Ichi Hayashi17. Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic YieldLesley A. Davis Anke Dienelt and Nicole I. zur Nieden18. Identification of Osteoclasts in CultureNobuyuki Udagawa Teruhito Yamashita Yasuhiro Kobayashi and Naoyuki Takahashi19. Analysis of Glycosaminoglycans in Stem Cell GlycomicsBoyangzi Li Haiying Liu Zhenqing Zhang Hope E. Stansfield Jonathan S. Dordick and Robert J. Linhardt20. Drill Hole Defects: Induction Imaging and Analysis in the RodentAndre Obenaus and Pedro Hayes21. Measurement and Illustration of Immune Interaction after Stem Cell TransplantationStephan Fricke