Epidermal Growth Factor Methods And Protocols by Tarun B. Patel , Paul J. Bertics , Springer

Description

Springer Epidermal Growth Factor Methods And Protocols by Tarun B. Patel , Paul J. Bertics

A comprehensive collection of optimized methods for dissecting the mechanisms that control epidermal growth factors (EGF) and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls._x000D_ _x000D_A Historical Perspective of the EGF Receptor and Related Systems_x000D_ Francis Edwin, Gregory J. Wiepz, Rakesh Singh, Chimera Rene Peet, Deepti Chaturvedi, Paul J. Bertics, and Tarun B. Patel_x000D_ _x000D_ Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromatography_x000D_ Gregory J. Wiepz, Arturo G. Guadaramma, David L. Fulgham, and Paul J. Bertics_x000D_ _x000D_ Soluble Epidermal Growth Factor Receptor Acridinium-Linked Immunosorbent Assay_x000D_ Jacqueline M. Lafky, Andre T. Baron, and Nita J. Maihle_x000D_ _x000D_ Analysis of EGF Receptor Interactions With the a Subunit of the Stimulatory GTP Binding Protein of Adenylyl Cyclase, Gs_x000D_ Deepti Chaturvedi, Francis Edwin, Hui Sun, and Tarun B. Patel_x000D_ _x000D_ Regulator of Epidermal Growth Factor Signaling: Sprouty_x000D_ Esther Sook Miin Wong and Graeme R. Guy_x000D_ _x000D_ Dissecting the Epidermal Growth Factor Receptor Signal Transactivation Pathway_x000D_ Oliver M. Fischer, Stefan Hart, and Axel Ullrich_x000D_ _x000D_ A Sensitive Method to Monitor Ectodomain Shedding of Ligands of the Epidermal Growth Factor Receptor_x000D_ Umut Sahin, Gisela Weskamp, Yufang Zheng, Valerie Chesneau, Keisuke Horiuchi, and Carl P. Blobel_x000D_ _x000D_ In Vitro and In Vivo Assays of Monoubiquitination of Receptor Tyrosine Kinases_x000D_ Menachem Katz, Yaron Mosesson, and Yosef Yarden_x000D_ _x000D_ Assays to Monitor Degradation of the EGF Receptor_x000D_ Mirko H. H. Schmidt and Ivan Dikic_x000D_ _x000D_ Assessment of Degradation and Ubiquitination of CXCR4, a GPCR Regulated by EGFR Family Members_x000D_ Adriano Marchese_x000D_ _x000D_ Epithelial Cell Migration in Response to Epidermal Growth Factor_x000D_ Reema Zeineldin and Laurie G. Hudson_x000D_ _x000D_ Motility Signaled From the EGF Receptor and Related Systems_x000D_ Alan Wells, Brian Harms, Akihiro Iwabu, Lily Koo, Kirsty Smith, Linda Griffith, and Douglas A. Lauffenburger_x000D_ _x000D_ Methods for Determining the Proliferation of Cells in Response to EGFR Ligands_x000D_ Gregory J. Wiepz, Francis Edwin, Tarun B. Patel, and Paul J. Bertics_x000D_ _x000D_ Clinical Advancement of EGFR Inhibitors in Cancer Therapy_x000D_ Prakash Chinnaiyan and Paul M. Harari_x000D_ _x000D_ Index_x000D_