Description
Springer Experimental And Translational Methods To Screen Drugs Effective Against Seizures And Epilepsy by Divya Vohora
This volume discusses the latest experimental and clinical approaches to study seizures and epilepsy. Chapters in this book cover detailed protocols for ex vivo and in vitro experimental models and widely-used in vivo (acute and chronic seizure) models in epilepsy research. Chapters also cover techniques to screen the effective leads for the treatment of refractory seizures of pharmacoresistant epilepsy and invertebrate, non-mammalian models. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Experimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy is a valuable resource for researchers, in both industry and academia, interested in learning about this important and developing field. Series Preface...Foreword...Preface...Table of Contents...Contributing Authors...Part I Models for Seizures and Epilepsy: An Overview1. Models of Seizures and Epilepsy: Important Tools in the Discovery and Evaluation of Novel Epilepsy Therapies Wolfgang LoescherPart II Ex Vivo and In Vitro Models2. Protocol for Rodent Organotypic Hippocampal Slice Culture Model for Ex Vivo Monitoring of EpileptogenesisShabnam Ghiasvand, Jing Liu, Md. Joynal Abedin, and Yevgeny Berdichevsky3. Methods for the Screening of New Chemical Entities for Deciphering Neuroinflammatory and Associated Pathways in Seizures: An In Vitro PerspectivePreeti Vyas, Rajkumar Tulsawani, and Divya Vohora4. Cellular Electrophysiological Methods to Decipher the Altered Synaptic Transmission Associated with Drug Resistant EpilepsySoumil Dey, Aparna Banerjee Dixit, Manjari Tripathi, P. Sarat Chandra, and Jyotirmoy BanerjeePart III In Vivo Rodent Models 5. Acute Seizure Tests Used in Epilepsy Research: Step-by-Step Protocol of the Maximal Electroshock Seizure (MES) Test, The Maximal Electroshock Seizure Threshold (MEST) Test, and the Pentylenetetrazole (PTZ)-Induced Seizure Test in RodentsKatarzyna Socala and Piotr Wlaz6. Procedures for Electrical and Chemical Kindling Models in Rats and MiceHeidrun Potschka7. Methods for the Induction of status epilepticus and Temporal Lobe Epilepsy in Rodents: The Kainic Acid and the Pilocarpine ModelShreshta Jain, Nikita Nirwan, Fahim H. Pottoo, Nidhi Bharal Agarwal, and Divya Vohora 8. Seizure Monitoring in RodentsEdward H. Bertram Part IV Models for Refractory Seizures 9. Protocol for 6Hz Corneal Stimulation in Rodents for Refractory SeizuresRazia Khanam and Divya Vohora10. A Method to Induce Lamotrigine-Resistant Corneal Kindled MiceMelissa Barker-Haliski11. Protocol for Drug Screening with Quantitative Video-Electroencephalography in a Translational Model of Refractory Neonatal SeizuresBrennan J. Sullivan and Shilpa D. KadamPart V Invertebrate/Non-Mammalian Models 12. Methods to Investigate Seizures and Associated Cognitive Decline using Zebrafish ModelBrandon Choo Kar Meng and Mohd. Farooq Shaikh13. C. elegans as a Potential Model for Acute Seizure-Like ActivityAlistair Jones, Anthony G. Marson, Vincent T. Cunliffe, Graeme J. Sills, and Alan MorganSubject Index List...