Description
Humana Functional Genomics Methods And Protocols Vol. 224 by Michael J. Brownstein , Arkady Khodursky
This collection of robust, readily reproducible methods for microarray-based studies includes expert guidance in the optimal data analysis and informatics. On the methods side are proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering. Fabrication of cDNA MicroarraysCharlie C. Xiang and Michael J. BrownsteinNylon cDNA Expression ArraysGeorge Jokhadze, Stephen Chen, Claire Granger, and Alex ChenchikPlastic Microarrays: A Novel Array Support Combining the Benefits of Macro- and Microarrays, Alexander MunishkinKonrad Faulstich, Vissarion Aivazachvili, Claire Granger, and Alex ChenchikPreparing Fluorescent Probes for Microarray StudiesCharlie C. Xiang and Michael J. BrownsteinEscherichia coli Spotted Double-Strand DNA Microarrays: RNA Extraction, Labeling, Hybridization, Quality Control, and Data ManagementArkady B. Khodursky, Jonathan A. Bernstein, Brian J. Peter, Virgil Rhodius, Volker F. Wendisch, and Daniel P. ZimmerIsolation of Polysomal RNA for Microarray AnalysisYoav AravaParallel Analysis of Gene Copy Number and Expression Using cDNA MicroarraysJonathan R. PollackGenome-wide Mapping of Protein-DNA Interactions by Chromatin Immunoprecipitation and DNA Microarray Hybridization,Jason D. LiebStatistical Issues in cDNA Microarray Data AnalysisGordon K. Smyth, Yee Hwa Yang, and Terry SpeedExperimental Design to Make the Most of Microarray StudiesM. Kathleen KerrStatistical Methods for Identifying Differentially Expressed Genes in DNA MicroarraysJohn D. Storey and Robert TibshiraniDetecting Stable Clusters Using Principal Component AnalysisAsa Ben-Hur and Isabelle GuyonClustering in Life SciencesYing Zhao and George KarypisA Primer on the Visualization of Microarray DataPaul FawcettMicroarray Databases: Storage and Retrieval of Microarray DataGavin Sherlock and Catherine A. BallIndex