Description
Taylor & Francis G ProteinsTechniques of Analysis 1999 Edition by David R. Manning
Incorporating a bench-top format, G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility.G Proteins: Techniques of Analysis includes expression and functional analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and, mapping pathways established among receptors, G proteins, and effectors.Including contributors from key institutions, each author offers clear instructions to establish a synthesized, concise and consistent approach to each chapter, beneficial to students and professionals as well. Table of Contents : PrefaceExpression and Purification of G Protein aSubunits in E. scherichia coli - M. LinderPurification of Recombinant G Protein a and bg Subunits from Sf9 Cells-T. KozasaExpression and Functional Analysis of G Protein a Subunits in Mammalian Cells...-C. H. BerlotIdentification of Regulators and Targets of G Protein a Subunits Using the Yeast Two-Hybrid System-J.L. Glick, T. E. Meigs, and P. J. CaseyFunctional Analysis of G Protein bg Subunits...-K. Yan, O. Kisselev, A. Pronin and N. Gautam Immunological Techniques of Analysis-C. A. Chen and D. R. ManningGTPase-Activating Proteins (GAPs) for Heterotrimeric G Proteins- J. Wang, Y. Tu, S. Mukhopadhyay, P. Chidiac, G. Biddlecome and E. M. RossFatty Acid Acylation of G Protein a Subunits- P.B.WedegaetnerADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin- M. J. WoolkalisAnalysis of Receptor.G Protein coupling with PTX-Risistant Analogues of G(i in subscript) and G(o in subscript)- A. Mortiz and R. TaussigMicroinjection of Antibodies Neutralizing G. Protein Function-V. J. LaMorte, J. R. Feramisco and J. L. MeinkothAgonist-Promoted (35S)GTPgS-Binding as a Probe of Receptor-G Protein Communication in Reconstituted Sf9 Cells- A.J. Barr and D. R. ManningMethods of Sequestering bg in the Intact Cell- G. J. Della Rocca, R. J. Lefkowitz and W. J. KochIn Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling via DNA/RNA Antisense Strategies-H. Wang, X. Liu, Z. Cheng, H. Ling Li and C. C. Malbon