Description
COLD SPRING HARBOR LABORATORY PRESS Gene Transfer Delivery And Expression Of Dna And Rna A Laboratory Manual by FRIEDMANN
Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery. 1. The Study of Communication, Gender, and Culture.2. Theoretical Approaches to Gender Development.3. The Rhetorical Shaping of Gender: Women's Movements in the United States.4. The Rhetorical Shaping of Gender: Men's Movements in the United States.5. Gendered Verbal Communication.6. Gendered Nonverbal Communication.7. Becoming Gendered: The Early Years.8. Gendered Education: Communication in Schools.9. Gendered Close Relationships.10. Gendered Organizational Communication.11. Gendered Media.12. Gendered Power and Violence.