Genotoxicity Assessment Methods and Protocols by Alok Dhawan, Mahima Bajpayee , Humana

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Humana Genotoxicity Assessment Methods and Protocols by Alok Dhawan, Mahima Bajpayee

Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls._x000D__x000D_Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences._x000D_ Table of contents : - _x000D_ Part I: Gene Mutation Assays_x000D_ _x000D_ 1. Bacterial Mutation Assays_x000D_ _x000D_ Errol Zeiger _x000D_ _x000D_ _x000D_ _x000D_ 2. In Vitro Mouse Lymphoma (L5178Y Tk+/- -3.7.2.C) Forward Mutation Assay_x000D_ _x000D_ Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi_x000D_ _x000D_ _x000D_ _x000D_ 3. Erythrocyte--Based Pig--a Gene Mutation Assay_x000D_ _x000D_ Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger_x000D_ _x000D_ _x000D_ _x000D_ 4. Detection of in vivo Mutation in the Hprt and Pig--a Genes of Rat Lymphocytes_x000D_ _x000D_ Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich_x000D_ _x000D_ _x000D_ _x000D_ 5. In vivo cII, gpt, and Spi- gene Mutation Assays in Transgenic Mice and Rats_x000D_ _x000D_ Mugimane G. Manjanatha, Xuefei Cao, Sharon D. Shelton, Roberta A. Mittelstaedt, and Robert H. Heflich_x000D_ _x000D_ _x000D_ _x000D_ Part II; Assays for Chromosomal Abnormalities_x000D_ _x000D_ 6. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests _x000D_ _x000D_ Pasquale Mosesso, Serena Cinelli, Adyapalam T. Natarajan, and Fabrizio Palitti_x000D_ _x000D_ _x000D_ _x000D_ 7. Analysis of Chromosome Aberrations in Somatic and Germ Cells of the Mouse_x000D_ _x000D_ Francesca Pacchierotti and Valentina Stocchi_x000D_ _x000D_ _x000D_ _x000D_ 8. Chromosomal Aberration Test in Human Lymphocytes_x000D_ _x000D_ Christian Johannes and Guenter Obe_x000D_ _x000D_ _x000D_ _x000D_ 9. In vivo Micronucleus Assay in Mouse Bone Marrow and Peripheral Blood_x000D_ _x000D_ Sawako Kasamoto, Shoji Masumori, and Makoto Hayashi_x000D_ _x000D_ _x000D_ _x000D_ 10. Micronucleus Assay in Human Cells: Lymphocytes and Buccal Cells _x000D_ _x000D_ Claudia Bolognesi and Michael Fenech_x000D_ _x000D_ _x000D_ _x000D_ 11. Flow Cytometric Determination of Micronucleus Frequency_x000D_ _x000D_ Azeddine Elhajouji and Magdalena Lukamowicz-Rajska_x000D_ _x000D_ _x000D_ _x000D_ 12. Fluorescent in situ Hybridization (FISH) Technique for the Micronucleus Test_x000D_ _x000D_ Ilse Decordier and Micheline Kirsch-Volders_x000D_ _x000D_ 13. Comparative Genomic Hybridization (CGH) Genotoxicology_x000D_ _x000D_ Baumgartner A_x000D_ _x000D_ 14. The in vitro Micronucleus Assay and Kinetochore Staining: Methodology and Criteria for the Accurate Assessment of Genotoxicity and Cytotoxicity_x000D_ _x000D_ Bella B. Manshian, Neenu Singh, and Shareen H. Doak_x000D_ _x000D_ _x000D_ _x000D_ Part III: Tests for Primary DNA Damage_x000D_ _x000D_ 15. g--H2AX Detection in Somatic and Germ Cells of Mice_x000D_ _x000D_ Eugenia Cordelli and Lorena Paris_x000D_ _x000D_ _x000D_ _x000D_ 16. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers_x000D_ _x000D_ Julian Laubenthal, Michal R. Gdula , Alok Dhawan, and Diana Anderson_x000D_ _x000D_ _x000D_ _x000D_ 17. The Comet Assay: Assessment of in vitro and in vivo DNA Damage_x000D_ _x000D_ Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan_x000D_ _x000D_ _x000D_ _x000D_ 18. The Comet Assay in Human Biomonitoring_x000D_ _x000D_ Diana Anderson, Alok Dhawan, and Julian Laubenthal_x000D_ _x000D_ _x000D_ _x000D_ 19. The Comet assay in Marine Animals_x000D_ _x000D_ G. Frenzilli and B.P. Lyons_x000D_ _x000D_ _x000D_ _x000D_ 20. Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo_x000D_ _x000D_ Fabrice Nesslany_x000D_ _x000D_ _x000D_ _x000D_ 21. 32P--Postlabeling Analysis of DNA Adducts_x000D_ _x000D_ Heinz H. Schmeiser, Marie Stiborova, and Volker M. Arlt_x000D_ _x000D_ _x000D_ _x000D_ Part IV: Assays in Plants and Aleternate Animal Models_x000D_ _x000D_ 22. Micronucleus Assay with Tetrad Cells of Tradescantia_x000D_ _x000D_ Miroslav Misik, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, and Siegfried Knasmueller_x000D_ _x000D_ _x000D_ _x000D_ 23. The Wing--Spot and the Comet Tests as useful Assays Detecting Genotoxicity in Drosophila_x000D_ _x000D_ Ricard Marcos and Erico R. Carmona_x000D_ _x000D_ _x000D_ _x000D_ Part V: Guidelines_x000D_ _x000D_ 24. Genotoxicity Guidelines Recommended by International Conference of Harmonization (ICH)_x000D_ _x000D_ Gireesh Kamath H. and K.S. Rao_x000D_