Description
Satish Serial Publishing House Glimpse of Plant Tissue Culture and Molecular Biology by M Sivaji S Shakespear K Sakthivel
Plant tissue culture is a basic, fundamental science from the branch of plant biotechnology that helps in understanding the growth and development of plants at the cellular level. Mainly plant tissue culture is used for crop improvement either by developing new varieties through different techniques, such as somaclonal variation, protoplast fusion, etc., or genetic modification through transgenic technology i.e. the cells of the plants can be genetically altered and the modified cell will undergo for regeneration into whole plant with desirable characteristics through tissue culture technology. So, to develop transgenic crops with desirable traits, one should know thoroughly about tissue culture techniques and basics of molecular biology. This book deals with essential elements of Plant tissue culture techniques viz., Meristem culture, Haploid plant production, Somatic embryogenesis, Somoclonal variation, Protoplast fusion, Micropropagation, Secondary metabolite production and Germplasm conservation. In Molecular biology aspects, it covers Basics of nucleic acids, Gene structure, Central Dogma, Polymerase chain reaction, Sequencing and Blotting techniques, Recombinant technology, Cloning vectors, Plant transformation methods, Transgenic crops development for biotic, abiotic stress and quality improvement, DNA markers, DNA fingerprinting, Linkage and QTL analysis and Marker assisted selection. This book has been written in a very simple and crisp manner enabling easy understanding by the readers and students. This book also contains important objective type questions in each chapter at the end. This will be more useful for the students for their both University examination and competitive examination.