Description
Humana Press Inc. Hedgehog Signaling Protocols by Jamila I. Horabin
Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes._x000D_ _x000D_
TABLE OF CONTENTS_x000D_
Chapter 1. Purifying the Hedgehog protein and its variants by Darren P._x000D_
Baker, Frederick R. Taylor and R. Blake Pepinsky_x000D_
Chapter 2. Application of Sonic hedgehog to the developing chick limb_x000D_
by Eva Tiecke and Cheryll Tickle_x000D_
Chapter 3. Manipulation of Hedgehog signaling in Xenopus by means of_x000D_
embryo microinjection and application of chemical inhibitors by Thomas_x000D_
Hollemann, Emmanuel Tadjuidje, Katja Koebernick & Tomas Pieler_x000D_
Chapter 4. Isolation of rat telencephalic neural explants to assay Shh_x000D_
GABAergic interneuron differentiation-inducing activity by Rina Mady_x000D_
and Jhumku D. Kohtz_x000D_
Chapter 5. Genetic Analysis of the Vertebrate Hedgehog Signaling_x000D_
Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo by_x000D_
Sudipto Roy_x000D_
Chapter 6. Efficient Manipulation of Hedgehog/GLI Signaling using_x000D_
Retroviral Expression Systems by Maria Kasper, Gerhard Regl, Thomas_x000D_
Eichberger, Anna-Maria Frischauf and Fritz Aberger_x000D_
Chapter 7. Cell Surface Marker and Cell Cycle Analysis, Hedgehog_x000D_
Signaling, and Flow Cytometry by Kristina Detmer and Ronald E. Garner_x000D_
Chapter 8. Detecting tagged Hedgehog with Intracellular and_x000D_
Extracellular Immunocytochemistry for functional analysis by Ainhoa_x000D_
Callejo, Luis Quijada and Isabel Guerrero_x000D_
Chapter 9. Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled_x000D_
with Fluorescent in situ Hybridization of Hedgehog Target Genes by_x000D_
Armel Gallet and Pascal P. Therond_x000D_
Chapter 10. RNAi in the Hedgehog signaling pathway: pFRiPE, a vector_x000D_
for temporally and spatially controlled RNAi in Drosophila by Eric_x000D_
Marois and Suzanne Eaton_x000D_
Chapter 11. Germline clone analysis for maternally acting Drosophila_x000D_
Hedgehog components by Erica M. Selva and Beth E. Stronach_x000D_
Chapter 12. Clonal analysis of Hedgehog signaling in Drosophila somatic_x000D_
tissues by Christine M. Bankers and Joan E. Hooper_x000D_
Chapter 13. GAL4/UAS targeted gene expression for studyingDrosophila_x000D_
Hedgehog signaling by Denise Busson and Anne-Marie Pret_x000D_
Chapter 14. Biochemical Fractionation of Drosophila Cells by Melanie_x000D_
Stegman and David Robbins_x000D_
Chapter 15. Using Immunoprecipitation to Study Protein-protein_x000D_
Interactions in the Hedgehog Signaling Pathway by Chao Tong and Jin_x000D_
Jiang_x000D_
Chapter 16. Sequence analyses to study the evolutionary history and_x000D_
cis-regulatory elements of Hedgehog genes by Ferenc Muller and_x000D_
Anne-Gaelle Borycki_x000D_