Description
Humana Human Monoclonal Antibodies Methods And Protocols 2013 Edition by Michael Steinitz
The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production purification and application of human monoclonal antibodies as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting. Table of contents : 1. Human Monoclonal Antibodies - The Residual Challenge of Antibody ImmunogenicityHerman Waldmann2. Technical and Ethical Limitations in Making Human Monoclonal AntibodiesMark C. Glassy and Rishab Gupta3. Therapeutic Human Monoclonal Antibodies in Inflammatory DiseasesSotirios Kotsovilis and Evangelos Andreakos4. Therapeutic Human Monoclonal Antibodies Against CancerJamie Jarboe Anumeha Gupta and Wasif Saif5. Polyclonal and Monoclonal Antibodies in the ClinicBharath Wootla Aleksandar Denic and Moses Rodriguez6. Production of Human Monoclonal Antibodies by the Epstein- Barr Virus MethodMichael Steinitz7. Humanization and Simultaneous Optimization of Monoclonal AntibodyT. Kuramochi T. Igawa H. Tsunoda and K. Hattori8. Chimeric AntibodiesKohei Kurosawa Waka Lin and Kunihiro Ohta9. Recombinant Genetic Libraries and Human Monoclonal AntibodiesJarrett J. Adams Bryce Nelson and Sachdev S. Sidhu10. Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial CytoplasmLilach Vaks and Itai Benhar11. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody IgG-Single Chain Fv FusionDan Lu and Zhenping Zhu12. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage DisplayAndre Frenzel Jonas Kugler Sonja Wilke Thomas Schirrmann and Michael Hust13. Antigen -Specific Human Monoclonal Antibodies from Transgenic MiceSusana Magadan Mompo and Africa Gonzalez-Fernandez14. Phage Display Technology for Human Monoclonal AntibodiesCecilia Deantonio Diego Cotella Paolo Macor Claudio Santoro and Daniele Sblattero15. Antigen-Specific in vitro Immunization: A Source for Human Monoclonal AntibodiesKosuke Tomimatsu and Sanetaka Shirahata 16. Methods for Radiolabelling of Monoclonal AntibodiesVladimir Tolmachev Anna Orlova and Karl Andersson17. Purification of Human Monoclonal Antibodies and Their FragmentsThomas Muller-Spath and Massimo Morbidelli18. Idiotype-Specific IVIG for Therapy of Autoimmune DiseasesTomer Bashi Miri Blank and Yehuda Shoenfeld