Description
Humana Human Retroviruses Methods And Protocols 2013 Edition by Elisa Vicenzi Guido Poli
Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections this detailed volume covers mapping of the HIV life cycle isolation co-receptor use and cell tropism of HIV-1 in vivo quantification of HIV-1 biological aspects of HIV-1 as well as HTLVs. Some articles explore "assay and function of accessory genes" largely involving the interface between retroviral and host factors the extracellular role of Tat and Tax resembling the function of cytokines and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology essential for any lab working this vital field. Table of contents : Part I: Mapping the HIV Life Cycle1. Virion Attachment & Entry: HIV gp120 Env Biotinylation gp120 Env or Integrin Ligand Binding AssayClaudia Cicala and James Arthos2. CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor TRIM5 Gongpu Zhao and Peijun Zhang3. The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 UncoatingYang Yang Jeremy Luban and Felipe Diaz-Griffero4. The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected CellsThomas Hope5. Imaging HIV-1 Nuclear Pre-Integration ComplexesAnna Cereseto and Mauro Giacca6. HIV-1 Reverse TranscriptionAndrea Cimarelli and Jean-Luc Darlix7. RNase H: Specificity Mechanisms of Action and Antiviral TargetKarin Moelling Felix Broecker and John E. Kerrigan8. HIV-1 Chromatin Transcription and the Regulatory Protein TatLaurence Colin Eric Verdin and Carine Van Lint9. HIV-1 Rev Function and RNA Nuclear-Cytoplasmic ExportAlan Cochrane10. HIV-1 Accessory Proteins: NefAnke Heigele Daniel Sauter Jan Munch and Frank Kirchhoff11. HIV-1 Accessory Proteins: VpRRichard Y. Zhao and Michael I. Bukrinsky12. HIV-1 Accessory Proteins: Vpu and VifAmy Andrew and Klaus Strebel13. SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid CellsGregory Berger and Andrea Cimarelli14. Imaging of HIV Assembly and ReleaseBarbara Muller and Jacomine Krijnse-LockerPart II: Isolation Co-Receptor Use and Cell Tropism of HIV-115. HIV-1 Isolation from Infected Peripheral Blood Mononuclear CellsStefania Dispinseri Elisa Saba Elisa Vicenzi Neeltje A. Kootstra Hanneke Schuitemaker and Gabriella Scarlatti16. Determination of HIV-1 Co-Receptor UsageMariangela Cavarelli and Gabriella Scarlatti17. The Macrophage and HIV: Basic Concepts and MethodologiesSuzanne Gartner18. HIV Infection of Dendritic CellsNajla Nasr Andrew Harman Stuart Turville and Anthony L. Cunningham19. Histocultures (Tissue Explants) in Human RetrovirologyAnush Arakelyan Wendy Fitzgerald Jean-Charles Grivel Christophe Vanpouille and Leonid MargolisPart III: In Vivo Quantification of HIV-120. Single Copy Quantification of HIV-1 in Clinical SamplesAnn Wiegand and Frank Maldarelli21. Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear CellsChristine Rouzioux Adeline Melard and Veronique Avettand-FenoelPart IV: Biotechnological Aspects of HIV-122. HIV-1 Based Lentiviral VectorsYing Poi Liu and Ben Berkhout23. Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular CloneZachary A. Klase Laurent Houzet and Kuan-Teh JeangPart V: HTLVs24. Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic TechniquesGreta Forlani Roberto S. Accolla and Giovanna Tosi25. Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCRClaudio Casoli Elisabetta Pilotti and Umberto Bertazzoni26. Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR)Ilaria Cavallari Francesca Rende and Vincenzo Ciminale