Description
SPRINGER Immunity In Insects (Pb 2020) by SANDRELLI F.
This volume details methods and protocols necessary to further the study of insect immunity. Chapters guide readers through up-to-date genomic and transcriptomic approaches, insect samples for proteomic analysis, hemocytes in Drosophila, cellular response in Lepidoptera, insect AMPs, manipulate the composition of mosquito microbiota, viral infections in insects, infections by entomopathogenic nematodes, immune response following oral infections, and protocols to to monitor the effect of septic infections with human pathogens using B. mori as a model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Immunity in Insects aims to ensure successful results in the further study of this vital field. Part I: Searching for Genes and Proteins Involved in the Immune Response1. Characterization of Insect Immune Systems from Genomic DataRobert M. Waterhouse, Brian P. Lazzaro, and Timothy B. Sackton2. Analyzing Immunity in Non-model Insects using de novo TranscriptomicsShulin He, Paul R. Johnston, and Dino P. McMahon3. Preparation of Insect Protein Samples for Label-free Proteomic Quantification by LC-mass SpectrometryAlexandro Rodriguez-Rojas and Jens RolffPart II: Cellular Response4. Quantification of Blood Cells in Drosophila melanogaster and other InsectsSean Corcoran and Katja Bruckner5. Methods to Quantify in vivo Phagocytic Uptake and Opsonization of Live or Killed microbes in Drosophila melanogasterSamuel Liegeois, Wenhui Wang, and Dominique Ferrandon6. Analysis of Cellular Immune Responses in Lepidopteran LarvaeAndrea Becchimanzi, Ilaria Di Lelio, Francesco Pennacchio, and Silvia Caccia Part III: Humoral Response: Melanization, Lysozyme, and Antimicrobial Peptides7. Detection of Enzyme Distribution, Expression, Activation, and Activity of Insect ProphenoloxidaseKai Wu, Bing Yang, Jing Wang, Yufa Luo, Yuyang Ni, and Wuren Huang8. Basic Methods to Evaluate Humoral Immunity Processes in Lepidoptera larvaeMaristella Mastore and Maurizio Francesco BrivioPart IV: Humoral Response: Antimicrobial Peptides as Alternatives to Conventional Antibiotics9. Methods for the in vitro Examination of the Antibacterial and Cytotoxic Activities of Antimicrobial PeptidesBruno Casciaro, Floriana Cappiello, Maria Rosa Loffredo, and Maria Luisa Mangoni10. Characterization of Antimicrobial Peptide - Membrane Interaction Using All-atom Molecular Dynamic SimulationShruti Mukherjee, Rajiv K. Kar, Anirban BhuniaPart V: Insect Immunity and Environmental Interactions 11. Manipulating the Mosquito Microbiota to Study its FunctionOttavia Romoli and Mathilde Gendrin12. Discovery and Analysis of RNA Viruses in Insects Lumi Viljakainen and Jaana JurvansuuVI: Infection Models 13. A Workflow for Infection of Drosophila with Entomopathogenic Nematodes to Monitor Immune Gene Transcriptional ActivityChrista Heryanto, Eric Kenney, and Ioannis Eleftherianos14. Oral Infection in a Germ-Free Bombyx mori Model Daniel Brady, Alessio Saviane, Ottavia Romoli, Gianluca Tettamanti, Federica Sandrelli, and Silvia Cappellozza15. Silkworm Infection Model for Evaluating Pathogen VirulenceYasuhiko Matsumoto and Kazuhisa Sekimizu