Ion Channels Methods And Protocols by James D. Stockand , Mark S. Shapiro , Springer

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Springer Ion Channels Methods And Protocols by James D. Stockand , Mark S. Shapiro

The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation._x000D_ _x000D_Part I. Methods for Exogenous Expression of Ion Channels in Cells_x000D_ _x000D_ Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System_x000D_ Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D. Stockand, and Qiusheng Tong_x000D_ _x000D_ Overexpression of Proteins in Neurons Using Replication-Deficient Virus_x000D_ Richard M. Ahlquist and Jane M. Sullivan_x000D_ _x000D_ Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System_x000D_ Nikita Gamper and Mark S. Shapiro_x000D_ _x000D_ Part II. Methods for Studying Channel Structure-Function_x000D_ _x000D_ Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels_x000D_ John M. Robinson, Andrey Kosolapov, and Carol Deutsch_x000D_ _x000D_ Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System_x000D_ Farhad Kosari, Shaohu Sheng, and Thomas R. Kleyman_x000D_ _x000D_ Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis_x000D_ Jie Zheng_x000D_ _x000D_ Part III. Methods for Studying Channel Regulation and Physiological Function_x000D_ _x000D_ Probing the Effects of Phosphoinositides on Ion Channels_x000D_ Chou-Long Huang_x000D_ _x000D_ Epithelial Sodium Channel in Planar Lipid Bilayers_x000D_ Bakhrom K. Berdiev and Dale J. Benos_x000D_ _x000D_ A Simple In Vivo Method for Assessing Changes of Membrane- Bound Ion Channel Density in Xenopus Oocytes_x000D_ Mouhamed S. Awayda, Weijian Shao, Ivana Vukojicic, and Abderrahmane Bengrine_x000D_ _x000D_ Preparation of Cortical Brain Slices for Electrophysiological Recording_x000D_ Costa M. Colbert_x000D_ _x000D_ Juxtacellular Labeling and Chemical Phenotyping of Extracellularly Recorded Neurons In Vivo_x000D_ Glenn M. Toney and Lynette C. Daws_x000D_ _x000D_ Carbon Fiber Amperometry in the Study of Ion Channels and Secretion_x000D_ Duk-Su Koh_x000D_ _x000D_ Part IV. Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology_x000D_ _x000D_ Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K+ Uptake-Deficient Escherichia coli_x000D_ Lyubov V. Parfenova and Brad S. Rothberg_x000D_ _x000D_ KCNQ1 K+ Channel-Mediated Cardiac Channelopathies_x000D_ Gildas Loussouarn, Isabelle Baro, and Denis Escande_x000D_ _x000D_ Tissue-Specific Transgenic and Knockout Mice_x000D_ Andree Porret, Anne-Marie Merillat, Sabrina Guichard, Friedrich Beermann, and Edith Hummler_x000D_ _x000D_ Index_x000D_