Description
Humana Press Inc. Lipid Rafts by Thomas J. McIntosh
There are many unanswered questions concerning the composition, structure, and dynamics of lateral microdomains (rafts) in biological membranes. Thus, sophisticated techniques have been used to study intact cell membranes as well as model systems composed of specific lipids and proteins thought to be in rafts. Using biochemical, biophysical, and computational methods, "Lipid Rafts" provides the most up-to-date information on the subject._x000D_ _x000D_
TABLE OF CONTENTS_x000D_
'Lipid Rafts'_x000D_
Volume for Methods in Molecular Biology_x000D_
Chapters:_x000D_
1. 'Overview of Membrane Rafts' by Thomas J. McIntosh, Duke University_x000D_
Medical Center._x000D_
2. 'Analysis of Raft Affinity of Membrane Proteins by Detergent_x000D_
Insolubility' by Deborah A. Brown, SUNY Stony Brook_x000D_
3. 'Non-detergent Isolation of Rafts' by Mehul Shah and Pravin Sehgal,_x000D_
New York Medical College._x000D_
4. 'Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes_x000D_
Using Tempo' by Omar Bakht and Erwin London, SUNY Stony Brook_x000D_
5. 'Using Monomolecular Films to Characterize Lipid Lateral_x000D_
Interactions' by Rhoderick E. Brown and Howard L. Brockman, University_x000D_
of Minnesota_x000D_
6. 'Electro-formation and Fluorescence Microscopy of Giant Vesicles_x000D_
with Coexisting Liquid Phases' by Sarah Veatch, University of British_x000D_
Columbia._x000D_
7. 'Fluorescence Correlation Spectroscopy' by Kirsten Bacia and Petra_x000D_
Schwille, Dresden University._x000D_
8. 'Multiphoton Laser Scanning Microscopy and Spatial Analysis of_x000D_
Dehydroergosterol Distribution on Plasma Membrane of Living Cells' by_x000D_
Avery McIntosh, Barbara Atshaves, Adalberto M. Gallegos, Ann B. Kier,_x000D_
Friedhelm Schroeder, Hai Xu, Weimin Zhang, Suojin Wang, and Jyh-Charn_x000D_
Liu, Texas A&M University._x000D_
9. 'NMR Detection of Lipid Domains' by Klaus Gawrisch, NIAAA, NIH_x000D_
10. 'Lateral Diffusion Coefficients of Raft Lipids from Pulsed Field_x000D_
Gradient NMR' by Greger Oradd and Goran Lindblom, Umea University._x000D_
11. 'Saturation-Recovery EPR Discrimination by Oxygen Transport Method_x000D_
for Characterizing Membrane Domains' by Witold K. Subczynski, Justyna_x000D_
Widomska, Anna Wisniewska, and Akihiro Kusumi, Medical College_x000D_
Wisconsin._x000D_
12. 'Plasmon-Waveguide Resonance Spectroscopy Studies of Lateral_x000D_
Segregation in Solid-Supported Proteolipid Bilayers' by Zdzislaw_x000D_
Salamon, Savitha Devanathan, and Gordon Tollin, University of Arizona._x000D_
13. 'Fluorescence Recovery after Photobleaching Studies ofLipid Rafts'_x000D_
by Anne Kenworthy, Vanderbilt_x000D_
14. 'Single Molecule Tracking' by Marija Vrljic, Stefanie Y. Nishimura,_x000D_
and W. E. Moerner, Stanford University_x000D_
15. 'X-ray Diffraction to Determine the Thickness of Raft and Non-raft_x000D_
Bilayers' by Thomas J. McIntosh, Duke University Medical Center._x000D_
16. 'Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains'_x000D_
by Jeremy Pencer, Thaila T. Mills, Norbert Kucerka, Mu-Ping Nieh, and_x000D_
John Katsaras, Canadian National Research Council_x000D_
17. 'Exploring Membrane Domains Using Native Membrane Sheets and_x000D_
Transmission Electron Microscopy' by Bridget S. Wilson, Janet R._x000D_
Pfeiffer, Mary Ann Raymond-Stintz, Diane Lidke, Nicholas Andrews, Jun_x000D_
Zhang, Wenxia Yin, Stanly Steinberg, and Janet M. Oliver, University of_x000D_
New Mexico._x000D_
18. 'Atomic Force Microscopy of Lipid Domains in Supported Model_x000D_
Membranes' by Alan Burns, Sandia National Laboratories._x000D_
19. 'Atomistic and Coarse-grained Computer Simulations of Raft-like_x000D_
Lipid Mixtures' by Sagar A. Pandit and Larry Scott, Illinois Institute_x000D_
of Technology_x000D_
20. 'A Microscopic Model Calculation of the Phase Diagram of Ternary_x000D_
Mixtures of Cholesterol and Saturated and Unsaturated Phospholipids' by_x000D_
R. Elliot, I. Szleifer, and M. Schick, UC Santa Barbara, Purdue_x000D_
University, and University of Washington._x000D_