Description
Taylor & Francis Manual of Immunological Methods 1998 Edition by Canadian Networking
The Manual of Immunological Methods represents the collaboration of the Canadian Network of Toxicology Centers, a non-profit network of university-based scientists dedicated to research, training, risk assessment, and communication. This manual provides detailed immunological methods that can be utilized by researchers or practitioners who want to enhance the successful application of this science. It emphasizes the need for continuously improving the quality of experiments performed and maintaining consistency in the results obtained.The Manual of Immunological Methods presents validated assays that are accompanied by a series of tools that will guide readers in performing their own laboratory work in the spirit of the Good Laboratory Practices. It covers specific methodologies including sample collection, preparation of cells, assay conditions, and data evaluation. Discussion of species differences and comparisons of a wide variety of species is an important feature. Both graduate students and practicing researchers in the areas of immunology and immunotoxicology will appreciate the Manual of Immunological Methods as a guide to standard operating procedures in the laboratory. Table of Contents : Identification, Anaesthesia, and EuthanasiaIdentification of Laboratory Rodents by Ear NotchingIdentification of Fish by Fin ClippingAnaesthesia of Fish and RodentsEuthanasia of RodentsEuthanasia of FishWorking Sheet: Weight of Laboratory AnimalsCollection of Peripheral Blood SamplesReagentsMaterials and EquipmentProcedureRemoval of OrgansReagents Materials and EquipmentProcedurePreparation of Cell SuspensionsExtrusion of Coelomocytes in the EarthwormHemocyte Collection in the MolluskCell Suspension from Peritoneal ExudateIsolation of White Blood Cells from Peripheral BloodCell Suspensions from Lymphoid OrgansAssessment of Cell ViabilityDetermination of Cell Viability and Concentration by Trypan Blue Dye ExclusionDetermination of Cell Viability and Cell Concentration with Ethidium Bromide and Acridine OrangeFlow Cytometric Assessment of Cell ViabilityWorking Sheet: Cell Viability and Concentration by Trypan Blue Dye Exclusion or Ethidium Bromide/Acridine OrangeCell CryopreservationReagentsMaterials and EquipmentProcedureSpecial RecommendationsAnalysis of ResultsSpecies-Related Changes in the ProtocolTechnique-Related Changes in the Protocol: Phagocytosis with YeastStatistical TestsWorking Sheet: Phagocytic FunctionOxidative Burst Assay Using Flow CytometryReagentsMaterials and EquipmentProcedureSpecial RecommendationsAnalysis of ResultsSpecies-Related Changes in the ProtocolStatistical TestsWorking Sheet: Acquisition ListCell CytotoxicityNatural Killer (NK) Cell ActivityAntibody-Dependent Cellular CytotoxicityLymphokine-Activated Killer (LAK)Working Sheet: Acquistion ListLymphoblastic TransformationReagentsMaterials and EquipmentProcedureAnalysis of ResultSpecial RecommendationsSpecies-Related Changes in the ProtocolWorking Sheet: Mitogenic AssayDetermination of Antibody-Producing Cells to a Specific AntigenLiquid Plaque Forming Cell Assay in Mouse ModelAgar Plaque Forming Cell Assay in Rat ModelWorking Sheet: Plaque Forming Cells (PFC)Mixed Lymphocyte Reaction (MLR)ReagentsMaterials and EquipmentProcedureAnalysis of ResultsSpecial RecommendationsSpecies-Related Changes in the ProtocolWorking Sheet: Mixed Lymphocyte ReactionIntracellular Levels of Calcium Assay Using Flow CytometryReagentsMaterials and EquipmentProcedureSpecial RecommendationsAnalysis of ResultsSpecies-Related Changes in the ProtocolStatistical TestsWorking Sheet: Acquisition ListPhenotyping of Blood Mononuclear CellsReagentsMaterials and EquipmentProcedureSpecial RecommendationsAnalysis of ResultsSpecies-Related Changes in the ProtocolImmunophenotype of Rat Peripheral Blood LymphocytesWorking SheetEvaluation of Intracellular Level of ThiolsReagentsMaterials and EquipmentProcedureSpecial RecommendationsAnalysis of ResultsSpecies-Related Changes in the ProtocolWorking Sheet: Acquisition ListApoptosisReagentsMaterials and EquipmentProcedureAnalysis of Results