Description
Humana Mass Spectrometry In Metabolomics Methods And Protocols 2014 Edition by Daniel Raftery
Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids cells or tissue plants foods and related samples and this information provides enormous detail on biological systems and their current status. Mass Spectrometry in Metabolomics: Methods and Protocols presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics this volume includes a wide variety such as triple quads time of flight Fourier transform ion cyclotron resonance and even simple quadrupole systems. A wide range of studies are described with samples ranging from blood and urine to tissue and even plants. Written in the successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible Mass Spectrometry in Metabolomics: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics. Table of contents : Part I. Historical and Methodology Overview1. Overview: of Mass Spectrometry Based Metabolomics: Opportunities and ChallengesG. A. Nagana Gowda and Danijel DjukovicPart II. Global LC-MS Based Metabolic Profiling2. Global Metabolic Profiling using Liquid Chromatography Quadrupole Time-of-Flight Mass SpectrometryYunpeng Qi Yunlong Song Haiwei Gu Guorong Fan and Yifeng Chai3. LC-MS Profiling to Link Metabolic and Phenotypic Diversity in Plant Mapping PopulationsCamilla B. Hill Antony Bacic and Ute Roessner4. Mitochondrial Metabolomics Using High-Resolution Fourier-Transform Mass SpectrometryYoung-Mi Go Karan Uppal Douglas I. Walker ViLinh Tran Lauriane Dury Frederick H. Strobel Helene Baubichon-Cortay Kurt D. Pennell James R. Roede and Dean P. Jones5. Sample Preparation Methods for LC-MS Based Global Aqueous Metabolite Profiling Antoni Beltran Sara Samino and Oscar YanesPart III. Global GC-MS and CE-MS Based Metabolic Profiling6. Methods of Discovery-Based and Targeted Metabolite Analysis by Comprehensive Two-Dimensional Gas Chromatography with Time-of-Flight Mass Spectrometry DetectionLuke C. Marney Dr. Jamin C. Hoggard Kristen J. Skogerboe and Robert E. Synovec7. Analysis of Mouse Liver Metabolites by GCxGC-TOF MSXue Shi Xinmin Yin and Xiang Zhang8. Metabolite Fingerprinting by Capillary Electrophoresis-Mass SpectrometryAntonia Garcia Shama Naz and Coral BarbasPart IV. Isotope Labeling Methods9. Quantitative Metabolomic Profiling using Dansylation Isotope Labeling and Liquid Chromatography Mass SpectrometryRuokun Zhou and Liang Li10. Quantitative Analysis of Amino and Organic Acids by Methyl Chloroformate Derivatization and GC-MS/MS AnalysisHans Fredrik Nyvold Kvitvang Kare A. Kristiansen Stina K. Lien and Per Bruheim11. Stable Isotope Labeled Tracers for Metabolic Pathway Elucidation by GC-MS and FT-MSRichard M. Higashi Teresa W-M. Fan Pawel K. Lorkiewicz Hunter N.B. Moseley and Andrew N. LanePart V. Targeted Aqueous Metabolite Profiling12. Multiplexed Quantitative and Targeted Metabolite Profiling by LC-MS/MRMRu Wei Guodong Li and Albert B. SeymourPart VI. Lipid Profiling Methods13. Multi-Dimensional Mass Spectrometry-Based Shotgun LipidomicsMiao Wang and Xianlin Han14. Comprehensive Quantitative Determination of PUFA-Related Bioactive Lipids for Functional Lipidomics using High Resolution Mass SpectrometryMojgan Masoodi and Dietrich A. Volmer15. Ultra-Performance Liquid Chromatography Mass Spectrometry Targeted Profiling of Bile Acids: Application to Serum Liver Tissue and Cultured Cells of Different SpeciesJuan Carlos Garcia-Canaveras Maria Teresa Donato and Agustin LahozPart VII. Profiling Volatile Organic Compounds16. Analysis of Volatile Organic Compounds in Exhaled Breath by Gas Chromatography-Mass Spectrometry combined with Chemometric AnalysisJan W. Dallinga Agnieszka Smolinska and Frederik-Jan van Schooten17. Headspace SPME-GC-MS Metabolomics Analysis of Urinary Volatile Organic Compounds (VOCs)Shucha Zhang and Daniel RafteryPart VIII. Profiling and Imaging using Atmospheric Sampling Methods18. Metabolite Profiling by Direct Analysis in Real Time Mass SpectrometryChristina M. Jones Maria Eugenia Monge and Facundo M. Fernandez19. Analysis of Dried Blood Spots using DESI Mass SpectrometryJustin M. Wiseman and Joseph H. Kennedy20. DESI-MS Imaging of Lipids and Metabolites from Biological SamplesLivia S. Eberlin21. Metabolic Imaging using Nanostructure-Initiator Mass Spectrometry (NIMS)Katherine B. Louie and Trent R. NorthenPart IX. Statistical Analysis Methods22. Statistical Analysis and Modeling of Mass Spectrometry-Based Metabolomics Data Bowei Xi Haiwei Gu Hamid Baniasadi and Daniel Raftery