Description
Humana Molecular Diagnosis Of Infectious Diseases 2/E by Jochen Decker , Udo Reischl
Populations of the western world are now healthier and enjoying higher life expectancy than ever. They are beginning to benefit from an array of costly new therapies made possible through recent rapid advances in medical science and technology, and their demands on modern medicine are rising. Meanwhile, healthcare systems are struggling with their outdated legacy models of the m- th 20 century and are experiencing ever-increasing financial pressure from g- ernments and health insurance organizations. The equation is no longer in balance, and this predicament is forcing societies to explore new approaches to managing healthcare in the future. Since the first edition of Molecular Diagnosis of Infectious Diseases was published, we have witnessed the sequencing of the (almost) complete human genome and a shift in medical research from an emphasis on genetics to the advancement and useful application of proteomics. Bioinformatics has become the key tool for managing and analyzing the upsurge of data, and faster and more effective test methods and technologies have opened up new prospects for ind- try and academia. The tools of modern genomics and proteomics are now being utilized to specifically guide the discovery of drugs for the prevention, diagnosis, and treatment of human disease. They may also help us to find a way out of the current healthcare calamity. Part I. Protein-Based Identification of AntigensProteomic Approaches to Antigen DiscoveryKaren M. Dobos, John S. Spencer, Ian M. Orme, and John T. BelisleImmunoproteomicsAlexander Krah and Peter R. JungblutImmunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and LectinsStephen ThompsonPart II. DNA-Based Identification of AntigensRepresentational Difference Analysis of cDNALucas D. BowlerMicroarray Data Analysis and MiningSilvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, and Raffaele A. CalogeroExpression CloningMichael J. Lodes, Davin C. Dillon, Raymond L. Houghton, and Yasir A. W. SkeikyPart III. Identification of Epitopes and Immunomodulatory ComponentsDetermination of Epitopes by Mass SpectrometryChristine Hager-Braun and Kenneth B. TomerIdentification of T Cell Epitopes Using ELISpot and Peptide Pool ArraysTimothy W. Tobery and Michael J. CaulfieldVirus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte ActivityLudwig Deml, Jens Wild, and Ralf WagnerApplication of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial ComponentsLudwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, and Wulf Schneider-BrachertPart IV. Expression of Recombinant ProteinsHigh-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well FormatJutta Drees, Jason Smith, Frank Schafer, and Kerstin SteinertProduction of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant ProteinsMarkus FuhrmannCodon-Optimized Genes that Enable Increased Heterologous Expression inMammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNAMarcus Graf, Ludwig Deml, and Ralf WagnerPart V. Purification, Modification, and Renaturation of Recombinant ProteinsPurification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)Udo ReischlPurification of Recombinant Proteins with High Isoelectric PointsRaffaele A. Calogero and Anna AulicinoRefolding of Inclusion Body ProteinsMarcus Mayer and Johannes BuchnerSmall-Molecule-Protein Conjugation ProceduresStephen ThompsonPart VI. Characterization of Recombinant ProteinsStructural Characterization of Proteins and PeptidesRainer DeutzmannDetermination of Kinetic Data Using Surface Plasmon Resonance BiosensorsClaudia Hahnefeld, Stephan Drewianka, and Friedrich W. HerbergAffinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) BiosensorUwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, and Hans WolfPart VII. Evaluation of Recombinant Proteins in Immunological Test SystemsSolid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase ImmunoassaysJohn E. ButlerDesign and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent AssaysAlan Warnes, Anthony R. Fooks, and John R. StephensonBasic Problems of Serological Laboratory DiagnosisWalter FierzMolecular Diagnostics Resources on the InternetLarry WingerPart VIII. Recombinant Receptor MoleculesCloning Single-Chain Antibody Fragments (scFv) from Hybridoma CellsLars Toleikis, Olaf Broders, and Stefan DubelIndex