Description
Wiley India Molecular Methods In Plant Biology A Comprehensive Book On Biotechnological Aspects by Krishnananda Pralhad Ingle and Mangesh Pradip Moharil
This book integrates the experimental procedures and theoretical principles for undergraduate, postgraduate, academicians and researchers in the area of agriculture and life sciences. The experiments have been updated and extended to reflect developments in the respective fields. In-text worked examples are again used to enhance student's understanding of each topic. The book is designed to provide students with the experience of how scientists use their knowledge to understand real-world science related issues that confront them in their professional lives.
About the Author
Krishnananda Pralhad Ingle is an Associate Scientist, Jain Irrigation Systems Ltd., Jalgaon. He also served as an editorial board member of Acta Scientific Journal of Agriculture (International) and Science Publishing Group (USA). He has a vast experience in the Molecular Breeding and Biotechnological aspects. He has worked for esteem organizations like MAHYCO, India Ltd. (Jalna); VNR seeds Pvt. Ltd. (Raipur); and Biostadt MH Seeds Ltd. (Hyderabad).
Dipika Ashok Padole :- is Junior Research Assistant, Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India. She has a vast experience in the field of microbial study, insect molecular biotechnology, genetic transformation studies, and plant tissue culture. She has submitted Gene Bank accessions for Helicoverpa chitin synthase B gene at NCBI, USA. She has a vast experience in the Plant Tissue Culture and other biotechnological aspects.
Mangesh Pradip Moharil :- is Associate Professor of Biotechnology, Department of Agricultural Botany, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, India. He is a member of the Society of Biotechnologist (India), and Microbiologist Society (Maharashtra), India. He has a vast experience in the field of Molecular Biology and has 14 years of teaching experience in the area of Molecular Biology, Genomics and Proteomics, Microbial Biotechnology, Genomics and Proteomics, and Entomological Research.
TABLE OF CONTENTS
BOOK REVIEW
Isolation of Plant Genomic DNA
Isolation of Plasmid DNA
Polyacrylamide Gel Electrophoresis
Polymerase Chain Reaction (PCR) Amplification
RNA Isolation Using TRIzol Method
Expression Analysis Using Semi-Quantitative Real Time-PCR
Quantitative Expression Analysis by Real Time-PCR
Analysis of DNA Sequences by Southern Blotting and Hybridization
Analysis of DNA Sequences by Northern Blotting and Hybridization
Histochemical GUS Assay
Enzyme-Linked Immunosorbent Assay (ELISA)
DNA Gel Extraction Protocol
Competent Cell Preparation
Ligation, Transformation and Colony PCR
DNA Sequencing and Analysis
Glossary
Instruments Used in the Laboratory
Appendix