Description
SPRINGER Molecular Toxicology Protocols 3Ed (Pb 2020) by KEOHAVONG P.
This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines. Part I: Bioinformatics and Biostatistics1. Bioenergetic Analyses of In Vitro and In Vivo Samples to Guide Toxicological EndpointsJonathan W. Boyd, Julia A. Penatzer, Nicole Prince, Julie V. Miller, Alice A. Han, and Holly N. Currie2. Next Generation Sequencing (NGS) Application in Multi-Parameter Gene Expression AnalysisDegeng Wang and Andrey L. Karamychev3. Integrated Chip-Seq and RNA-Seq Data Analysis Coupled with Bioinformatics Approaches to Investigate Regulatory Landscape of Transcription Modulators in Breast Cancer CellsJairo Ramos, Quentin Felty, and Deodutta Roy4. Analysis of RNA Sequencing Data using CLC Genomics WorkbenchChia-Hsin Liu and Y. Peter DiPart II: Toxicant-Induced Lung Inflammation and Lung Tumors5. Using Bronchoalveolar Lavage to Evaluate Changes in Pulmonary DiseasesMarissa E. Di, Dandan Yang, and Y. Peter Di6. Fast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing SystemsYingze Zhang, Xiaoyun Li, and Y. Peter Di7. Induction of Lung Tumors and Mutational Analysis in FVB/N Mice Treated with the Tobacco Carcinogen 4-(Methylnitrosamino)-1-(3-pyridyl)-1-ButanoneNatalie J. Rothenberger and Laura P. StabilePart III: Analysis of Protein and Gene Expression and Silencing8. Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein AnalysisWeimin Gao9. Determination and Quantification of Bacterial Virulent Gene Expression Using Quantitative Real Time PCRQiao Lin and Y. Peter Di10. Using a Lentivirus-Based Inducible RNAi Vector to Silence a GeneSi Chen, Dongying Li, Zhen Ren, Dianke Yu, Baitang Ning, Nan Mei, and Lei GuoPart IV: Gene Promoter Methylation11. Analysis of Toxicants-Induced Alterations in DNA Methylation by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR)Kamaleshwar P. Singh12. Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of TechniquesHang-Kai Hsu, Yu-I Weng, Pei-Yin Hsu, Tim H.-M. Huang, and Yi-Wen HuangPart V: Detection of Chromosomal and Genome-Wide Damage13. Analysis of Telomere Length and Aberrations by Quantitative FISHElise Fouquerel and Patricia Opresko14. Detection of Loss of Heterozygosity in Tk-Deficient Mutants from L5178Y Tk+/--3.7.2C Mouse Lymphoma CellsXiaoqing Guo, Ying Chen, Martha M. Moore, and Nan Mei15. Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCRSheetal Singh, Shyh-Jen Shih, and Andrew T. M. VaughanPart VI: Analysis of DNA-Adducts and Surrogate Gene Mutations16. 32P-Postlabeling Analysis of DNA AdductsDavid H. Phillips and Volker M. Arlt17. Determination of Mutational Spectra Induced by Environmental Toxicants in Complex Human Cell PopulationsRobert Gealy and Phouthone Keohavong18. Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and MiceVasily N. Dobrovolsky, Xuefei Cao, Javed A. Bhalli, and Robert H. Heflich19. Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse LymphocytesVasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich20. Molecular Analysis of Mutations in the Human HPRT GenePhouthone Keohavong, Liqiang Xi, and Stephen G. Grant21. Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions In Human LymphocytesJames C. FuscoePart VII: Detection and Characterization of Cancer Gene Mutation22. Analysis of Mutations in K-ras and p53 Genes in Sputum and Plasma SamplesLifang Zhang, Wei-Min Gao, and Phouthone Keohavong23. ACB-PCR Quantification of Low-Frequency Hotspot Cancer-Driver MutationsMeagan B. Myers, Karen L. McKim, Yiying Wang, Malathi Banda, and Barbara L. Parsons24. Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic LesionsToshinari MinamotoPart VIII: Analysis of DNA Damage and Repair Mechanisms25. The Sister Chromatid Exchange (SCE) AssayDawn M. Stults, Michael W. Killen, Paola Marco-Casanova, and Andrew J. Pierce26. The Gene Cluster Instability (GCI) Assay for RecombinationMichael W. Killen, Dawn M. Stults, Paola Marco-Casanova, and Andrew J. Pierce27. New Perspectives on Unscheduled DNA Synthesis: Functional Assay for Global Genomic DNA Nucleotide Excision RepairManasi R. Pimpley, Megan L. Foley, and Jean J. Latimer28. Host Cell Reactivation: Assay for Actively Transcribed DNA Nucleotide Excision Repair Using Luciferase Family Expression VectorsJowaher S. Alanazi and Jean J. LatimerPart IX: Analysis of Endoplasmic Reticulum Stress and miRNA/RNA Interaction29. Methods for Establishing and Using a Stable Cell Line Expressing Both Gaussia Luciferase and Firefly Luciferase to Screen for Endoplasmic Reticulum StressZhen Ren, Si Chen, and Lei Guo30. FREMSA: A Method That Provides Direct Evidence of the Interaction Between MicroRNA and mRNADianke Yu, Si Chen, Dongying Li, Bridgett Knox, Lei Guo, and Baitang Ning