Description
Springer Myeloid Leukemia Methods And Protocols by Harry Iland , Mark Hertzberg , Paula Marlton
A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered._x000D_ _x000D_Isolation of RNA and DNA From Leukocytes and cDNA Synthesis_x000D_
Joop H. Jansen and Bert A. van der Reijden_x000D_
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Cytogenetic and FISH Techniques in Myeloid Malignancies_x000D_
Lynda J. Campbell_x000D_
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Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies_x000D_
Christophe Picard, Monique Silvy, and Jean Gabert_x000D_
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Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR_x000D_
Susan Branford and Timothy Hughes_x000D_
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Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate_x000D_
Susan Branford and Timothy Hughes_x000D_
_x000D_
Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia_x000D_
Lynda J. Campbell_x000D_
_x000D_
Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR_x000D_
Vincenzo Rossi, Laura Levati, and Andrea Biondi_x000D_
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Diagnosis and Monitoring of PML-RARa-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR_x000D_
Elisa Mokany, Alison V. Todd, Caroline J. Fuery, and Tanya L. Applegate_x000D_
_x000D_
Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR_x000D_
Khalid Tobal and John A. Liu Yin_x000D_
_x000D_
Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR_x000D_
Bert A. van der Reijden and Joop H. Jansen_x000D_
_x000D_
Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia_x000D_
Jan Cools, Elizabeth H. Stover, and D. Gary Gilliland_x000D_
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FLT3 Mutations in Acute Myeloid Leukemia_x000D_
Hitoshi Kiyoi and Tomoki Naoe_x000D_
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WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes_x000D_
Daniela Cilloni, Enrico Gottardi, and Giuseppe Saglio_x000D_
_x000D_
Classification of AML by DNA-Oligonucleotide Microarrays_x000D_
Alexander Kohlmann, Wolfgang Kern, Wolfgang Hiddemann, and Torsten Haferlach_x000D_
_x000D_
Classification of AML Using a Monoclonal Antibody Microarray_x000D_
Richard I. Christopherson, Kerryn Stoner, Nicole Barber, Larissa Belov, Adrian Woolfson, Mike Scott, Linda Bendall, and Stephen P. Mulligan_x000D_
_x000D_
Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders_x000D_
Peter J. Campbell, Linda M. Scott, E. Joanna Baxter, Anthony J. Bench, Anthony R. Green, and Wendy N. Erber_x000D_
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Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia_x000D_
Maurizio Martini, Luciana Teofili, and Luigi M. Larocca_x000D_
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Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation_x000D_
Thomas Lion and Franz Watzinger_x000D_
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Index_x000D_