Description
Humana Nucleic Acid Detection Methods and Protocols by Dmitry M. Kolpashchikov, Yulia V. Gerasimova
In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls._x000D__x000D__x000D__x000D_Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA._x000D_ Table of contents : - _x000D_
Part I: Advances in Sample Preparation_x000D_
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1. "Getting Blood from a Stone": Ultra-Sensitive Forensic DNA Profiling of Microscopic Bio-particles Recovered from 'Touch DNA' Evidence_x000D_
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Erin K. Hanson and Jack Ballantyne_x000D_
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2. Detecting MicroRNA in Human Cancer Tissues with Fluorescence in situ Hybridization _x000D_
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Zonggao Shi, Jeff J. Johnson, and M. Sharon Stack_x000D_
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Part II: New Real-Time and Instantaneous Assays_x000D_
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3. MN Azyme qPCR: A Superior Tool for Multiplex qPCR_x000D_
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Elisa Mokay and Alison V. Todd_x000D_
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4. A New, Multiplex, Quantitative Real-time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification_x000D_
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Fang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson, Graeme Barnett, David Whiley, Theo Sloots, Simon R. Corrie, and Ross T. Barnard_x000D_
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5. Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with Universal Molecular Beacon Reporter_x000D_
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Yulia V. Gerasimova, Jack Ballantyne, and Dmitry M. Kolpashchikov_x000D_
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6. SNP Analysis using a Molecular Beacon-based Operating Cooperatively (OC) Sensor _x000D_
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Evan M. Cornett and Dmitry M. Kolpashchikov_x000D_
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Part III: Isothermal Amplification Methods for Nucleic Acid Detection_x000D_
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7. Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification_x000D_
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Wanyuan Ao and Robert Jenison _x000D_
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8. Rapid Detection of Brucella spp. using Loop-mediated Isothermal Amplification (LAMP)_x000D_
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Shouyi Chen, Xunde Li, Juntao Li, and Edward R. Atwill_x000D_
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9. Loop-mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms_x000D_
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Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, and Akira Ito_x000D_
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10. Detection of Mutation by Allele-specific Loop-mediated Isothermal Amplification (AS-LAMP)_x000D_
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Hiroka Aonuma, Athanase Badolo , Kiyoshi Okado, and Hirotaka Kanuka_x000D_
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Part IV: Signal Amplification Approac_x000D_
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11. DNA Detection by Cascade Enzymatic Signal Amplification _x000D_
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Bingjie Zou, Yinjiao Ma and Guohua Zhou_x000D_
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Part V: Non-Fluorescent Detection Formats_x000D_
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12. Visual DNA Detection and SNP Genotyping using Asymmetric PCR and Split DNA Enzymes_x000D_
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Jia Ling Neo and Mahesh Uttamchandani _x000D_
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13. Detection of Single-stranded Nucleic Acids via Colorimetric Means, using G-quadruplex Probes_x000D_
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Shizuka Nakayama and Herman O. Sintim_x000D_
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14. Lateral Flow Biosensors for the Detection of Nucleic Acid_x000D_
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Lingwen Zeng, Puchang Lie, Zhiyuan Fang, Zhuo Xiao_x000D_
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15. Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a _x000D_
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Wireless Bipolar Electrode_x000D_
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Mei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu, and Hong-Yuan Chen_x000D_
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16. High-throughput Label-free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor_x000D_
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Sunmin Ahn, David S. Freedman, Xirui Zhang, and M. Selim UEnlu1_x000D_
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Part VI: Advances in Fish_x000D_
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17. Graphene -PAMAM Dendrimer -Gold Nano Particle Composite for Electrochemical DNA Hybridization Detection _x000D_
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Kumarasamy Jayakumar , Rajendiran Rajesh ,Venkataraman Dharuman, and Rangarajan Venkatesan_x000D_
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18. Application of PNA Openers for Fluorescence-based Detection of Bacterial DNA_x000D_
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Irina Smolina_x000D_
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19. DNA probes for FISH analysis of C-Negative Regions in Human Chromosomes _x000D_
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Evgeniy S. Morozkin, Tatyana V. Karamysheva, Pavel P. Laktionov, Valentin V. Vlassov, and Nikolay B. Rubtsov_x000D_
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Part VII: Detection of microRNA_x000D_
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20. Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis_x000D_
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Nasrin Khan and Maxim V. Berezovski_x000D_
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21. High-Throughput Functional MicroRNA Profiling using Recombinant AAV-Based MicroRNA Sensor Arrays _x000D_
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Wenhong Tian, Xiaoyan Dong, Xiaobing Wu, and Zhijian Wu_x000D_
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22. The Use of Molecular Beacons to Detect and Quantify MicroRNA_x000D_
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Meredith B. Baker, Gang Bao, and Charles D. Searles_x000D_
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Part VIII: RNA Imaging in Live Cells_x000D_
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23. Sequence Specific Imaging of Influenza A mRNA in Living Infected Cells Using Fluorescent FIT-PNA_x000D_
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Susann Kummer, Andrea Knoll, Andreas Herrmann, and Oliver Seitz_x000D_
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24. Application of Caged Fluorescent Nucleotides to Live-cell RNA Imaging_x000D_
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Akimitsu Okamoto_x000D_