Description
Springer Periodontal Pathogens Methods And Protocols 2021 Edition by Nagano K.
This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. _x000D_Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it._x000D_ Table of contents :- Part I: Methods for Bacterial Genetic Manipulation1. Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene MutantSo-ichiro Nishiyama, Yoshiaki Hasegawa, and Keiji Nagano2. Genetic Manipulations of Oral Spirochete Treponema denticolaKurni Kurniyati and Chunhao Li3. Construction of a Gene-Deletion Mutant in Tannerella forsythiaKeiji Nagano and Yoshiaki Hasegawa4. Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coliYoshio Kondo5. Genetic Transformation of Fusobacterium nucleatumAkihiro Yoshida and Akihiko IkegamiPart II: Experimental Methods to Examine Virulence Factors6. Genotyping of Porphyromonas gingivalis in Relationship to VirulenceAtsuo Amano, Youn-Hee Choi, and Hiroki Takeuchi7. Transport and Polymerization of Porphyromonas gingivalis Type V PiliMikio Shoji, Satoshi Shibata, Mariko Naito, and Koji Nakayama8. Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalisYoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, and Richard J. Lamont9. Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalisThomas Heidler and Karina Persson10. Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalisTomoko Kadowaki11. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalisDhana G. Gorasia, Eric Hanssen, Paul D. Veith, and Eric C. Reynolds12. Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalisKeiko Sato13. Purification of Tannerella forsythia Surface (S-) Layer ProteinsSreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, and Ashu Sharma14. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythiaYukitaka Murakami, Keiji Nagano, and Yoshiaki Hasegawa15. Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse ModelSatoru Hirayama and Ryoma Nakao16. Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalisYasuo Yoshida17. Characterization of the Treponema denticola Virulence Factor DentilisinYuichiro Kikuchi and Kazuyuki Ishihara18. Evaluation of the Virulence of Aggregatibacter actinomycetemcomitans through the Analysis of LeukotoxinToshiyuki Nagasawa, Satsuki Kato, and Yasushi Furuichi19. Lipoprotein Extraction from Microbial Membrane and Lipoprotein/Lipopeptide Transfection into Mammalian CellsAkira Hasebe, Ayumi Saeki, and Ken-ichiro ShibataPart III: Interactions with Other Pathogenic Microorganism and Host Cells20. Analysis of the Interaction between HIV and Periodontopathic Bacteria that Reactivates HIV Replication in Latently Infected CellsKenichi Imai21. Invasion of Gingival Epithelial Cells by Porphyromonas gingivalisHiroki Takeuchi and Atsuo Amano22. Analysis of Interaction between Porphyromonas gingivalis and Endothelial Cells In VitroKenji MatsushitaPart IV: Animal Model of Periodontitis23. Analysis of Experimental Ligature-Induced Periodontitis Model in MiceHikaru Tamura, Tomoki Maekawa, Takumi Hiyoshi, and Yutaka Terao