Description
Humana Press Inc Placenta And Trophoblast Methods And Protocols Volume Ii by Michael J. Soares , Joan S. Hunt
The aim of the two-volume set of Placenta and Trophoblast: Methods and Protocols is to offer contemporary approaches for studying the biology of the placenta. The chapters contained herein also address critical features of the female organ within which the embryo is housed, the uterus, and some aspects of the embryo-fetus itself, particularly those of common experimental animal models. In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty. In both volumes, sources of supplies are given and illustrations highlight particular techniques as well as expected outcomes. A key aspect of these volumes is that the contributors are at the forefronts of their disciplines, thus ensuring the accuracy and usefulness of the chapters. Placenta research has progressed rapidly over the past several decades by taking advantage of the technical advances made in other fields. For example, the reader will note that many techniques, such as reverse transcriptase po- merase chain reaction, northern and western blotting, microarray analyses and in situ hybridization experiments, are routinely used for dissecting a wide range of experimental questions. Protein analysis and functional experiments on tissues and cells that comprise the maternal-fetal interface benefit from studies in en- crinology, immunology, and developmental biology. These volumes also present new ideas on investigating gene imprinting and gene transfer via viral vectors. IntroductionConnie M. Weaver and Robert P. HeaneyI. Calcium FunctionsBone as the Calcium Nutrient ReserveRobert P. HeaneyCellular Functions and Fluxes of CalciumEmmanuel M. Awumey and Richard D. BukoskiII. Techniques for Studying Calcium Metabolism and Its Relationship to DiseaseNutritional Epidemiology: Dietary Assessment MethodsCarol J. BousheyClinical Approaches for Studying Calcium Metabolism and Its Relationship to DiseaseConnie M. WeaverKinetic StudiesMeryl E. Wastney, Yongdong Zhao, and Connie M. WeaverIII. Calcium Consumption, Requirements, and BioavailabilityRequirements for What Endpoint?Robert P. Heaney and Connie M. WeaverDietary Calcium: Recommendations and Intakes Around the WorldAnne C. LookerFood Sources, Supplements, and BioavailabilityConnie M. Weaver and Robert P. HeaneyIV. Calcium HomeostasisThe Calcium EconomyRobert P. HeaneyMolecular Regulation of Calcium MetabolismJames C. FleetInfluence of Total Diet on Calcium HomeostasisZamzam K. (Fariba) RougheadInfluence of Lifestyle Choices on Calcium Homeostasis: Smoking, Alcohol, and Hormone TherapiesD. Lee Alekel, and Oksana MatvienkoInfluence of Physical Activity on Calcium and BoneMatthew Vukovich and Bonny SpeckerThe Case for a Calcium Appetite in HumansMichael G. TordoffV. Calcium Through DevelopmentInfancy and ChildhoodSteven A. Abrams and Keli M. HawthornePre-Puberty and AdolescenceConnie M. WeaverCalcium in Pregnancy and LactationHeidi J. KalkwarfVI. Calcium and DiseaseCalcium in Systemic Human HealthRobert P. HeaneyCalcium and Oral HealthElizabeth A. KrallDietary Calcium and ObesityDorothy TeegardenPolycystic Ovary Syndrome and ReproductionSusan Thys-JacobsPremenstrual SyndromeSusan Thys-JacobsCalcium Throughout the Life Cycle: The Later YearsBess Dawson-HughesCalcium, Vitamin D, and CancerPeter R. HoltDietary Calcium and the Metabolic SyndromeDorothy TeegardenCalcium and Phosphate Control in Patients With Renal DiseaseDavid A. McCarron and Robert P. HeaneyHypertension and Cardiovascular DiseaseDavid A. McCarronAppendicesIndex