Description
Humana Press Placenta and Trophoblast Methods and Protocols Volume II 1st Editon 2014 Softbound by Michael J. Soares, Joan S. Hunt
The aim of the two-volume set of Placenta and Trophoblast: Methods and Protocols is to offer contemporary approaches for studying the biology of the placenta. The chapters contained herein also address critical features of the female organ within which the embryo is housed, the uterus, and some aspects of the embryo–fetus itself, particularly those of common experimental animal models. In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty. In both volumes, sources of supplies are given and illustrations highlight particular techniques as well as expected outcomes. A key aspect of these volumes is that the contributors are at the forefronts of their disciplines, thus ensuring the accuracy and usefulness of the chapters. Placenta research has progressed rapidly over the past several decades by taking advantage of the technical advances made in other fields. For example, the reader will note that many techniques, such as reverse transcriptase po- merase chain reaction, northern and western blotting, microarray analyses and in situ hybridization experiments, are routinely used for dissecting a wide range of experimental questions. Protein analysis and functional experiments on tissues and cells that comprise the maternal–fetal interface benefit from studies in en- crinology, immunology, and developmental biology. These volumes also present new ideas on investigating gene imprinting and gene transfer via viral vectors. Placenta and Trophoblast: Methods and Protocols: Overview II.- Analysis of Trophoblast Invasion.- In Vivo Analysis of Trophoblast Cell Invasion in the Human.- In Vitro Analysis of Trophoblast Invasion.- An In Vitro Model of Trophoblast Invasion of Spiral Arteries.- Analysis of Uteroplacental Immune Cells and Their Functions.- In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells.- Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface.- Methods for Isolation of Cells from the Human Fetal-Maternal Interface.- In Vitro Models for Studying Human Uterine and Placental Macrophages.- Macrophage-Trophoblast Interactions.- Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon.- Analysis of the Soluble Isoforms of HLA-G.- Analysis of Placental Function.- In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport.- In Vitro Models for Studying Trophoblast Transcellular Transport.- In Vitro Methods for Studying Human Placental Amino Acid Transport.- In Vitro Methods for Studying Human Placental Amino Acid Transport.- Methods for Investigating Placental Fatty Acid Transport.- Heterologous Expression Systems for Studying Placental Transporters.- Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures.- Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells.- Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands.- Bacterial Expression of Prolactin Family Proteins.- Analysis of Placental Regulation of Hematopoiesis.- Methods for Studying Interferon Tau Stimulated Genes.- ANALYSIS OF PLACENTA ADAPTATION TO DISEASE.- Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia.- In Vivo Rat Model of Preeclampsia.- A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice.- Working with Oxygen and Oxidative Stress In Vitro.- Hypobaric Hypoxia as a Tool to Study Pregnancy-Dependent Responses at the Maternal-Fetal Interface.- Infection With Listeria monocytogenes as a Probe for Placental Immunological Function.