Description
SPRINGER Plant Cell Division Methods And Protocols 2Ed (Hb 2022) by CAILLAUD M.C.
This new edition explores innovative approaches and keystone methodologies reflecting the recent advances in the field of plant cell division that have enabled us to study this fascinating process in a quantitative manner, at high resolution both in space and time using cell biology, biochemistry, and molecular biology. After a review of the methods used to visualize the actin cytoskeleton during plant cell division, the book focuses first on methodology to address mitosis progression as a part of the cell cycle. It continues with sections on manipulation of cell division, quantification of cell division patterning, as well as imaging and quantifying plant cytokinesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cell Division: Methods and Protocols, Second Edition serves as an ideal guide for researchers attempting to visualize, quantify, and modify cell division during cell cycle progression. 1. Methods to Visualize the Actin Cytoskeleton during Plant Cell DivisionMarie-Cecile CaillaudPart I: Mitosis and Cell Cycle Progression2. Immunolabeling of Nuclei/Chromosomes in Arabidopsis thalianaUlkar Ahmadli, Michael Sandmann, Joerg Fuchs, and Inna Lermontova3. A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root TipsLeigh Mickelson-Young, Emily E. Wear, Jawon Song, Gregory J. Zynda, Linda Hanley-Bowdoin, and William F. Thompson4. Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal CulturesKaterina Bisova5. Analysis of Commitment Point Attainment in Algae Dividing by Multiple FissionVeronika Kselikova, Vilem Zachleder, and Katerina BisovaPart II: Manipulation of Cell Division6. Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical Stage Confocal MicroscopyLukas Hoermayer, Jiri Friml, and Matous Glanc7. Transient Overexpression of E2Fb to Induce Cell Divisions in Nicotiana benthamiana Pavement CellsTamara Jimenez-G ngora, Huang Tan, and Rosa Lozano-DuranPart III: Quantification of Cell Division Patterning8. Kinematic Growth Analysis of Rice (Oryza sativa) LeafJingjing Fang and Xueyong Li9. Studying Cell Division Plane Positioning in Early-Stage EmbryosKatia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia10. Means to Quantify Vascular Cell File Numbers in Different TissuesHelena E. Arents, Gugan Eswaran, Matous Glanc, Ari Pekka Mahoenen, and Bert De Rybel11. Confocal Analysis of Arabidopsis Root Cell Divisions in 3D: A Focus on the EndodermisRoya Campos and Jaimie M. Van Norman12. Quantification of Cell Division Angles in the Arabidopsis RootKatia Belcram, David Legland, and Martine PastugliaPart IV: Imaging and Quantifying Plant Cytokinesis13. A Quantitative Method for Evaluating Phragmoplast MorphologyTakema Sasaki and Yoshihisa Oda14. Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching during CytokinesisAlexandra Deli, Leda-Eleni Tympa, and Panagiotis N. Moschou15. Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 CellsKeisho Maeda and Takumi Higaki16. Ultrastructural Observation of Cytokinesis and Plasmodesmata Formation in Brown AlgaeChikako Nagasato, Rina Yonamine, and Taizo Motomura