Description
Springer Plant Lipids Methods And Protocols (HB) by Bartels D.
This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally. Table of Contents:- Preface...Table of Contents...Contributing Authors...Part I Basic Methods of Lipid Isolation and Analysis1 Lipid Isolation from PlantsJesse D. Bengtsson, James G. Wallis, and John Browse2. Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and YieldSamira Mahboub, Zachery D. Shomo, R. Maxwell Regester, Mahaa Albusharif, and Rebecca L. Roston3. Thin-Layer Chromatography Georg Hoelzl and Peter Doermann4. Lipid Analysis by Gas Chromatography and Gas Chromatography Mass SpectrometryMathias Brands, Philipp Gutbrod, and Peter Doermann5. 14C-Tracing of Lipid MetabolismHari Kiran Kotapati and Philip D. Bates6. Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and EuglenophytesMasako Iwai, Shiori Shibata, Hiroyuki Ohta, and Koichiro AwaiPart II Mass Spectrometry and NMR Analysis7. Direct Infusion Mass Spectrometry for Complex Lipid Analysis Katharina Gutbrod, Helga Peisker, and Peter Doermann8. Fatty Acid Composition by Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight (TAL-CID-TOF) Mass SpectrometryPamela Tamura, Carl Fruehan, David K. Johnson, Paul Hinkes, Todd D. Williams, and Ruth Welti 9. Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MSCornelia Herrfurth, Yi-Tse Liu, and Ivo Feussner10. Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant MetabolismRebecca E. Cahoon, Ariadna Gonzalez Solis, Jonathan E. Markham, and Edgar B. Cahoon11. Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MSRichard Broughton and Frederic Beaudoin12. Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and MicroalgaeRichard P. Haslam and Tony R. Larson13. Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS Lauren M. Jenkins, Jeong-Won Nam, Bradley S. Evans, and Doug K. Allen14. Structural Analysis of Glycosylglycerolipids using NMR SpectroscopyWiebke Knaack, Georg Hoelzl, and Nicolas GischPart III Lipid Isolation and Analysis from Plant Tissues Cell Compartments and Organelles 15. Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and SeedsJohanna Baales, Viktoria V. Zeisler-Diehl, and Lukas Schreiber 16. Isolation of Lipid Droplets for Protein and Lipid AnalysisPatrick J. Horn, Kent D. Chapman, and Till Ischebeck 17. Isolation of Plastoglobules for Lipid AnalysesDenis Coulon and Claire Brehelin18. Isolation of Mitochondria for Lipid AnalysisSebastien Leterme, Morgane Michaud, and Juliette Jouhet 19. Collection and Analysis of Phloem Lipids Susanne Hoffmann-BenningPart IV Lipid Signaling, Lipid-Protein Interactions, and Imaging20. Analyses of Inositol Phosphates and Phosphoinositides by Strong Anion Exchange (SAX)-HPLC Debabrata Laha, Marilia Kamleitner, Philipp Johnen, and Gabriel Schaaf21. Analysis of Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption Chromatography and Subsequent Quantification via Thin-Layer and Gas Chromatography Larissa Launhardt, Monique Matzner, Mareike Heilmann, and Ingo Heilmann22. Studying Lipid-Protein Interactions Using Protein-Lipid-Overlay and Protein-Liposome-Association AssaysGuido Ufer, Peter Doermann, and Dorothea Bartels23. Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC) Ze-Hua Guo and Mee-Len Chye24. In Situ Localization of Plant Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI)Drew Sturtevant, Mina Aziz, Trevor B. Romsdahl, Chase D. Corley, and Kent D. Chapman Part V Lipid Databases 25. Plant Lipid DatabasesPeter Doermann 26. Lipid Pathway Databases with a Focus on Algae Naoki Sato and Takeshi ObayashiSubject Index List...show more