Description
Springer Plant Mitochondria Methods And Protocols (HB) by Aken O.V.
This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Mitochondria: Methods and Protocols provides a highly useful set of methodologies for the plant mitochondrial community to help discover more interesting aspects of plant mitochondria in the years to come. Table of Contents:- 1. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density GradientsHuy Cuong Tran and Olivier Van Aken2. Cell Type-Specific Isolation of Mitochondria in ArabidopsisClement Boussardon and Olivier Keech3. Isolation of Plant Mitochondria Using Affinity PurificationFranziska Kuhnert and Andreas P.M. Weber4. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density GradientIan Max Moller and Allan G. Rasmusson5. Integrity Assessment of Isolated Plant MitochondriaAllan G. Rasmusson, Mengshu Hao, and Ian Max Moller6. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive FluorophoresBrendan M. O'Leary, Glenda Guek Khim Oh, and A. Harvey Millar7. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with AlamethicinAllan G. Rasmusson, Ian Max Moller, and Susanne Widell8. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS)Xuyen Le, A. Harvey Millar, and Chun Pong Lee9. Complexome Profiling of Plant Mitochondrial FractionsLucie Schroeder, Holger Eubel, and Hans-Peter Braun10. High Throughput BN-PAGE for Mitochondrial Respiratory ComplexesLilian Vincis Pereira Sanglard and Catherine Colas des Francs-Small11. Assessment of Mitochondrial Protein Composition and Purity by Mass SpectroscopyAnqiang Tang, Yimin Wang, and Nicolas L. Taylor12. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thalianaCristina Ruberti13. Assessment of Mitochondrial Protein Topology and Membrane InsertionKerstin Schafer, Carina Engstler, Korbinian Dischinger, and Chris Carrie14. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of ArabidopsisMalgorzata Kwasniak-Owczarek, Artur Tomal, and Hanna Janska15. Markers for Mitochondrial ROS StatusMonika Ostaszewska-Bugajska, Anna Podgorska, and Bozena Szal16. Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT LabelingJonas Giese, Jurgen Eirich, Frederik Post, Markus Schwarzlander, and Iris Finkemeier17. Analysis of the Plant Mitochondrial TranscriptomeKevin Baudry, Etienne Delannoy, and Catherine Colas des Francs-Small18. Quantification of Mitochondrial RNA Editing Efficiency Using Sanger Sequencing DataMizuki Takenaka19. Nascent Transcript Sequencing for the Mapping of Promoters in Arabidopsis thaliana MitochondriaOmar Saleh, Sarlita Dwiani, Julia Rott, and Kristina Kuhn20. Assessment of Mitochondrial DNA Copy Number, Stability, and Repair in ArabidopsisDeborah Schatz-Daas, Arnaud Fertet, Frederique Lotfi, and Jose M. Gualberto21. Measuring the Activity of DNA Repair Enzymes in Isolated MitochondriaBeatriz Ferrando, Ian Max Moller, and Tinna Stevnsner22. MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial GenomesShin-ichi Arimurashow more