Description
SPRINGER Plant Synthetic Biology Methods And Protocols (Hb 2022) by ZURBRIGGEN M.D.
This volume provides methods on different aspects and applications on plants, algae, photosynthetic bacteria, synthetic construct design, and multiplex cloning. Chapters cover multiple aspects of synthetic metabolic, photosynthetic systems, metabolic and signaling pathways, advanced engineering of metabolic networks, isolation of organelles and co-culture of microorganisms, and methods for the on command manipulation of the relative stability of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Synthetic Biology: Methods and Protocols aims to be a useful resource for both researchers starting to explore novel experimental avenues as well as for experts willing to expand their portfolio of tools and strategies. 1. Heterologous production of plant terpenes in the photosynthetic bacterium Rhodobacter capsulatusAnita Loeschcke, Jennifer Hage-Hulsmann, Katrin Troost, Vera Wewer, Karl-Erich Jaeger, and Thomas Drepper2. Design of multiplexing CRISPR/Cas9 constructs for plant genome engineering using the GoldenBraid DNA assembly standardVazquez-Vilar M, Juarez P, Bernabe-Orts JM, and Orzaez D3. pSHDY - a new tool for genetic engineering of cyanobacteriaAnna Behle and Ilka M. Axmann4. Cas9-mediated targeted mutagenesis in plantsQuentin M. Dudley, Oleg Raitskin, and Nicola J. Patron5. Generation of stable, light-driven co-cultures of cyanobacteria with heterotrophic microbes Amit K. Singh and Daniel C. Ducat6. Gene editing in green alga Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteinsSimon Kelterborn, Francisca Boehning , Heide Evers , Irina Sizova , Olga Baidukova, and Peter Hegemann7. Coexpression and reconstitution of enzymatic cascades in bacteria using UbiGate.Kathrin Kowarschik and Marco Trujillo8. Homo-FRET imaging to study protein-protein interaction and complex formation in plantsStefanie Weidtkamp-Peters, Stephanie Rehwald, and Yvonne Stahl9. Plant X-tender toolbox for the assembly and expression of multiple transcriptional units in plantsTjasa Lukan, Kristina Gruden and Anna Coll10.AGROBEST: a highly efficient Agrobacterium-mediated transient expression system in Arabidopsis seedlingsHung-Yi Wu and Erh-Min Lai11. Genetically-encoded biosensors for the quantitative analysis of auxin dynamics in plant cellsJennifer Andres and Matias D. Zurbriggen12. Exploiting the Gal4/UAS system as plant orthogonal molecular toolbox to control reporter expression in Arabidopsis protoplasts.Sergio Iacopino, Francesco Licausi, and Beatrice Giuntoli13. Mathematical Modelling in Plant Synthetic Biology Anna Deneer and Christian Fleck14. In vivo Epitope-tagging of Plant MitochondriaFranziska Kuhnert and Andreas P. M. Weber15. Engineering destabilizing N-termini in plastidsLioba Inken Winckler and Nico Dissmeyer16. Trichome transcripts as efficiency control for Synthetic Biology and Molecular FarmingRichard Becker, Christian Goerner, Pavel Reichman, and Nico Dissmeyer