Description
SPRINGER Protein Folding Methods And Protocols (Hb 2022) by MUNOZ V.
This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 1. Mutational Analysis of Protein Folding Transition States: Phi-valuesLuis Alberto Campos2. Engineered Metal-Binding Sites to Probe Protein Folding Transition States: Psi AnalysisMichael Baxa, Tobin R. Sosnick3. Site-Specific Interrogation of Protein Structure and Stability Debopreeti Mukherjee, Ismail A. Ahmed, and Feng Gai4. Purification and Handling of the Chaperonin GroEL Xiang Ye5. Folding free energy surfaces from Differential Scanning CalorimetryJose M. Sanchez-Ruiz and Beatriz Ibarra-Molero6. Fast Folding Kinetics using Nanosecond Laser-Induced Temperature Jump MethodsMichele Cerminara7. Measurement of Submillisecond Protein Folding using Trp Fluorescence and Photochemical OxidationDavid Witalka and Lisa J. Lapidus8. Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and UnfoldingPooja Malhotra and Jayant B. Udgaonkar9. Multi-Probe Equilibrium Analysis of Gradual (Un)Folding Processes Ginka S. Kubelka and Jan Kubelka10. Nmr Analysis Of Protein Folding Interaction NetworksEva de Alba11. NMR Relaxation Dispersion Methods for the Structural and Dynamic Analysis of Quickly Interconverting, Low-Populated Conformational Sub-StatesV. N. Sivanandam, Nicola D'Amelio, and Victor Munoz12. Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and UnfoldingPooja Malhotra and Jayant B. Udgaonkar13. Single-Molecule Fluorescence Spectroscopy Approaches for Probing Fast Biomolecular Dynamics and InteractionsZifan Wang, Nivin Mothi, and Victor Munoz14. Theory and Analysis of Single-Molecule FRET experimentsIrina V. Gopich and Hoi Sung Chung15. Mechanochemical Evolution Of Disulfide Bonds In ProteinsJoerg Schoenfelder, Alvaro Alonso-Caballero, and Raul Perez-Jimenez16. Coarse-Grained Simulations of Protein Folding: Bridging Theory and ExperimentsVinicius G. Contessoto, Vinicius M. Oliveira, and Vitor B. P. Leite17. Analysis of Molecular Dynamics Simulations of Protein FoldingRobert B. Best18. Atomistic Simulations of Thermal UnfoldingAngel E Garcia19. Molecular Simulations of Intrinsically Disordered Proteins and their Binding MechanismsXiakun Chu, Suhani Nagpal, and Victor Munoz20. Prediction of Folding and Unfolding Rates of Proteins with Simple ModelsDavid De Sancho and Victor Munoz21. Predicting and Simulating Mutational Effects on Protein Folding Kinetics Athi N. Naganathan22. Localization of Energetic Frustration in ProteinsA. Brenda Guzovsky, Nicholas P. Schafer, Peter G. Wolynes, and Diego U. Ferreiro23. Modeling the Structure, Dynamics, and Transformations of Proteins with the UNRES Force FieldAdam K. Sieradzan, Cezary Czaplewski, P awel Krupa, Magdalena A. Mozolewska, Agnieszka Karczynska, Agnieszka Lipska, Emilia A. Lubecka, Ewa Golas, Tomasz Wirecki, Mariusz Makowski, Stanislaw Oldziej, and Adam Liwo