Description
Humana Press Inc. Protein Nanotechnology Protocols Instrumentation and Applications 2020 Edition by Juliet A. Gerrard, Laura J. Domigan
This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field. Table of Content :- Preface...Table of Contents...Contributing Authors...1. Introduction to Protein NanotechnologyLaura J. Domigan and Juliet A. GerrardPart I Design and Production of Protein Building Blocks for Nanotechnology2. Protein Expression in the Baculovirus-Insect Cell Expression SystemAdetayo A. Adeniyi and Linda Hwee-Lin Lua3. Peroxiredoxin Proteins as Building Blocks for NanotechnologyFrankie Conroy and N. Amy Yewdall4. Formation of Amphipathic Monolayers from Fungal Hydrophobin ProteinsSarah R. Ball, Chi L. L. Pham, Victor Lo, Ann H. Kwan, and Margaret Sunde5. ATP Synthase: Expression, Purification, and FunctionMeghna Sobti, Robert Ishmukhametov, and Alastair G. Stewart6. Molecular Superglues: Discovery and Engineering OrthogonalizationPaul Young and Christopher Squires7. Production of Multicomponent Protein Templates for the Positioning and Stabilization of EnzymesSamuel Lim, Douglas S. Clark, and Dominic J. GloverPart II Towards Protein Nano-Devices8. Adding Function to Protein ScaffoldsKyle Webster, Luigi Sasso, and Laura J. Domigan9. Virus-Derived NanoparticlesNoor H. Dashti and Frank Sainsbury10. Strategies for Increasing Protein StabilityPeter G. Chandler, Sebastian S. Broendum, Blake T. Riley, Matthew A. Spence, Colin J. Jackson, Sheena McGowan, and Ashley M. Buckle11. Generation of High Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis ProtocolFrancesco Canfarotta, Sergey A. Piletsky, and Nicholas W. Turner12. Nanotechnology with S-Layer ProteinsBernhard Schuster and Uwe B. SleytrPart III Meaningful Measurements of Protein Nanocomponents13. Preparation of Proteins and Macromolecular Assemblies for Cryo-Electron MicroscopyLou Brillault and Michael J. Landsberg14. Atomic Force Microscopy of ProteinsYiran An, Sesha Sarathchandra Manuguri, and Jenny Malmstrom15. Native Protein Mass SpectrometryTimothy M. Allison and Mark T. Agasid16. Nanoparticle Tracking Analysis of b-Casein NanocarriersHikmat Masri and Jared K. Raynes17. Molecular Dynamics Simulation of ProteinsThomas A. Collier, Thomas J. Piggot, and Jane R. Allison