Description
HUMANA PRESS Protocols For Nucleic Acid Analysis By Nonradioactive Probes 2Nd Edition (Methods In Molecular Biology Volume 353) 2007 Edition by HILARIO ELENA ET. AL
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results.The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Contents(NOTE: Chapters not in MiMB/MiMM format are highlighted)PrefaceContributorsA. Nucleic Acid Extractions1. Genomic DNA Isolation from Different Biological MaterialsDuckchul Park2 Extraction of Plant RNAElspeth MacRaeB. Indirect Detection3 Overview on Hybridization and Detection TechniquesElena Hilario4 Checkerboard DNA:DNA Hybridization Technology using DIG DetectionGlenn Wall-Manning, Lisa S. Gellen, and Chris H. Sissons5 Non-radioactive Northern and Southern analyses from Plant SamplesChristoph Peterhaensel, Dagmar Weier, and Thomas Lahaye6 Screening a BAC library with Non-Radioactive Overlapping Oligonucleotide (Overgo) ProbesElena Hilario, Tiffany Bennell, and Erik Rikkerink7 Direct In-gel Hybridization of DNA with Digoxigenin-Labeled ProbesSaeed A. Khan and Mohamed S. Nawaz8 In situ Hybridization of Termite MicrobesShigeharu Moriya , Satoko Noda, Moriya Ohkuma and Toshiaki KudoC. Fluorescent Labeling and Detection9 RNA Electrophoretic Mobility Shift Assay using a Fluorescent DNA SequencerYukinori Eguchi10 Comparative Quantitation of mRNA Expression in the Central Nervous System using Fluorescence In situ HybridizationDarren Day, Eli M. Mrkusich, and John H. Miller11 Visualization of Gene Expression by Fluorescent Multiplex RT-PCR AmplificationMaria Rosa Ponce, Victor Quesada, Andrea Hricova, and Jose Luis Micol12 Fluorescence In situ Hybridization for the Identification of Environmental MicrobesAnnelie Pernthaler and Jakob PernthalerD. Kinetic ('Real-Time') PCR13 Introduction to Kinetic (Real-Time) PCRJohn Mackay14 Validation of siRNA Knockdowns by Quantitative Real-Time PCRSukru Tuzmen15 Real-Time Quantitative PCR as an Alternative to Southern Blot or FISH for Detection of Gene-Copy NumberChangesJasmien Hoebeeck, Frank Speleman, and Jo Vandesompele16 Design and Working up a New Molecular Diagnostic Assay Based on Real-Time PCRHarald Kessler17 Real-Time PCR Fluorescent ChemistriesJohn Mackay and Olfert LandtE. Microarrays18 Microarrays: An OverviewNorman H. Lee and Alexander I. Saeed19 Oligonucleotide Microarrays for the Study of Coastal Microbial CommunitiesGaspar Taroncher-Oldenburg and Bess B. Ward