Description
Humana Quantitative Real-Time Pcr Methods And Protocols 2014 Edition by Roberto Biassoni Alessandro Raso
Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material the knowledge of the minimal information required to both perform an assay and to set the experimental plan while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series most chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and authoritative Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation. Table of contents : 1. Twenty Years of qPCR: A Mature Technology?Alessandro Raso and Roberto Biassoni2. Minimum Information Necessary for Quantitative Real-Time PCR ExperimentsGemma Johnson Afif Abdel Nour Tania Nolan Jim Huggett and Stephen Bustin3. Selection of Reliable Reference Genes for RT-qPCR AnalysisJan Hellemans and Jo Vandesompele4. Introduction to Digital PCRFrancisco Bizouarn5. mRNA and micro RNA Purity and Integrity: The Key to Success in Expression ProfilingBenedikt Kirchner Vijay Paul Irmgard Riedmaier and Michael W. Pfaffl6. Mediator Probe PCR: Detection of Real-Time PCR by Label Free Probes and a Universal Fluorogenic ReporterSimon Wadle Stefanie Rubenwolf Michael Lehnert Bernd Faltin Manfred Weidmann Frank Hufert Roland Zengerle and Felix von Stetten7. Absolute Quantification of Viral DNA: The Quest for PerfectionDomenico Russo and Mauro Severo Malnati8. A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical SamplesFrancesco Broccolo9. Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of Community-Acquired PneumoniaEddi Di Marco10. A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP ConditionElisabetta Ugolotti and Irene Vanni11. Real-Time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid LeukemiaPierre Foskett Gareth Gerrard and Letizia Foroni12. A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCRSamantha Mascelli13. Circulating Cell-Free DNA in CancerPamela Pinzani Francesca Salvianti Claudio Orlando and Mario Pazzagli14. Gene Expression Analysis by qPCR in Clinical Kidney TransplantationMichael Eikmans Jacqueline D.H. Anholts and Frans H.J. Claas15. Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and Micro RNA DataSwanhild U. Meyer Katharina Stoecker Steffen Sass Fabian J. Theis and Michael W Pfaff16. Clinical Applications Using Digital PCRFrancisco Bizouarn17. Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCRAngela N. Barrett and Lyn S. Chitty