Description
Wiley India Rediscovering Genetics A Laboratory Manual by Sunita Joshi and Neeru Dhamija
Genetics is taught in all life science related disciplines at the undergraduate level. This laboratory manual meant for beginners is an outcome of the authors’ attempt made in the last three years (2012-15) of designing experiments to teach the principles of transmission genetics using Drosophila as a model system. The purpose of Drosophila Resource Centre was to demonstrate the ease of learning genetics through experimentation. Wherever required the existing approaches have also complemented the new effort.
About the Author
Dr. Sunita Joshi is presently an Associate Professor at Department of Biochemistry, Daulat Ram College, University of Delhi with more than 25 years of teaching experience. She has trained around 100 students from Botany, Biochemistry and Life Sciences in Drosophila Genetics in last three years. She has been a resource person in ILLL; DBT and CBSE sponsored workshops for teachers. She is a co-author of Textbook of Biotechnology, Class XI (CBSE).
Dr. Neeru Dhamija is presently an Assistant Professor at Department of Biochemistry, Daulat Ram College, University of Delhi. She has M.Sc. in Biochemistry and a doctorate in Biotechnology with specialization in HIV Biology. She studied the role of HIV-1 Tat protein in regulation of host cellular genes, under the guidance of Dr. Debashis Mitra at National Centre for Cell Science.
TABLE OF CONTENTS
BOOK REVIEW
Exercises
Maintenance and culturing of Drosophila
Sexing and collection of virgin females
To study the life cycle of Drosophila melanogaster
To study mutant phenotypes of Drosophila melanogaster
To demonstrate Mendel’s law of segregation and dominance
To demonstrate Mendel’s law of independent assortment
To study the inheritance of white eye colour in Drosophila
To map sepia and curl mutants by recombination mapping
To demonstrate absence of crossing over in Drosophila males
To establish dominance relationship between multiple alleles of a gene
To demonstrate that the white-eyed flies have a deposition defect
To perform cis-trans test of allelism with the given eye colour mutants
To demonstrate dosage compensation in Drosophila melanogaster
To create a Bar and apricot eyed double mutant
To detect recessive lethal alleles in genetic crosses
To study the penetrance and expressivity of serrate and vestigial wings in Drosophila melanogas
To study the characteristics of X-linked dominant traits
To separate eye pigments (pterins) by thin layer chromatography
To prepare stained squashes of salivary glands showing well spread polytene chromosomes
To demonstrate recombination within a gene
To determine the inheritance pattern of white eye colour and vestigial wings from the F2 data
To dissect the malphigian tubules, brain and imaginal discs from the third instar Drosophila larva
To analyse using bioinformatic tools Drosophila as a model system (Toll 9 gene) for human diseases (asthma)
To test for PTC tasting in a random population and to calculate allele and genotype frequency using Hardy Weinberg law
To determine the ABO blood groups in a random sample and calculate the allele frequency using Hardy Weinberg law
Appendix I
Appendix II