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Humana Renal Disease Techniques And Protocols by Michael S. Goligorsky
"Rule IV. There is need of a method for finding out the truth. Rule V. Method consists entirely in the order and disposition of the objects toward which our mental vision must be directed if we would find out any truth. We shall comply with it exactly if we reduce involved and obscure propositions step be step to those that are s- pler, and then starting with the intuitive apprehension of all those that are absolutely simple, attempt to ascend to the knowledge of all others by precisely similar steps. " -Rene Descartes, Rules for the Direction of Mind "...Perhaps he would sooner satisfy himself by resolving light into colours as far as may be done by Art, and then by examining the properties of those colours apart, and afterwards by trying the effects of reconjoyning two or more or all of those, and lastly by separating them again to examine what changes that reconjunction had wrought in them. This will prove a tedious and difficult task to do it as it ought to be done but I could not be satisfied till I had gone through it. " -From Newton's letter, quoted in The Life of Isaac Newton by Richard Westfall. Cambridge University Press, 1993. Part I. Optimizing the Usage of Models of Renal DiseaseStandards of Animal Care in Biological ExperimentsEllen M. LeveeModels of Polycystic Kidney DiseasePoornima UpadhyaRat Models of the Metabolic SyndromeStevan P. Tofovic and Edwin K. JacksonModels of GlomerulonephritisRaghu V. Durvasula and Stuart J. ShanklandPart II. Choices of Imaging Techniques in Studies of Renal DiseaseFunctional Studies of the Kidney with Magnetic Resonance ImagingHani B. Marcos, Yantian Zhang, and Peter L. ChoykeUse of Radionuclides to Study Renal FunctionYiyan Liu and M. Donald BlaufoxIntravital VideomicroscopyTokunori Yamamoto and Fumihiko KajiyaConfocal and Two-Photon MicroscopyJanos Peti-Peterdi and P. Darwin BellAtomic Force Microscopy in Renal PhysiologyRobert M. HendersonFreeze-Fracture Analysis of Renal-Epithelial Tight JunctionsHiroyuki SasakiPart III. Studies of Renal DevelopmentOrgan Culture of Intact Metanephric KidneysSimon J. M. Welham and Adrian S. WoolfThe Ureteric Bud: Tissue-Culture Approaches to Branching Morphogenesis and Inductive SignalingAlan O. PerantoniStudies of Cell Lineage in the Developing KidneyMaria Luisa S. Sequeira Lopez and R. Ariel GomezTransient Transfection Assays for Analysis of Signal Transduction in Renal CellsRobin L. Maser, Brenda S. Magenheimer, Christopher A. Zien, and James P. CalvetPart IV. Approaches to Study Molecular Mechanisms of DiseaseThe Study of Gene Polymorphisms: How Complex Is Complex Genetic Disease?Sylvia Bahring, Atakan Aydin, and Friedrich C. LuftLaser-Capture MicrodissectionLaura Barisoni and Robert A. StarSerial Analysis of Gene ExpressionM. Ashraf El-Meanawy, Shrinath Barathan, Patrick S.Hayden, Sudha K. Iyengar, Jeffrey R. Schelling, and John R. SedorRNA Labeling and Hybridization of DNA MicroarraysErwin P. Boettinger, Akiva Novetsky, and Jiri ZavadilGene-Expression Analysis of Microdissected Renal BiopsiesClemens D. Cohen and Matthias KretzlerThe Use of SELDI ProteinChip (R) Array Technology in Renal Disease ResearchEric Fung, Deb Diamond, Anja Hviid Simonsesn, and Scot R. WeinbergerPart V. Technical Means to Assess Functional Correlates of DiseaseClearance Studies in Genetically Altered MiceWilliam T. Noonan and John N. LorenzLong-Term Blood-Pressure Monitoring in Unrestrained AnimalsKaren A. Griffin, Isam Abu-Amarah, and Anil K. BidaniIn Vitro Studies on Renin ReleaseBoye L. Jensen, Ulla G. Friis, and Ole SkottMitochondrial FunctionJoel M. Weinberg and Pothana SaikumarDetection of Cysteine S-Nitrosylation and Tyrosine 3-Nitration in Kidney ProteinsMark Crabtree, Gang Hao, and Steven S. GrossProducts of Arachidonic Acid MetabolismMairead A. Carroll, John C. McGiff, and Nicholas R. FerreriMethods for Measurements of Heme Oxygenase (HO) Isoforms-Mediated Synthesis of Carbon Monoxide and HO-1 and HO-2 ProteinsNader G. Abraham, Houli Jiang, Michael Balazy, and Alvin I. GoodmanThe Juxtamedullary Nephron PreparationDaniel Casellas and Leon C. MooreTubuloglomerular FeedbackVolker Vallon and Jurgen SchnermannMicrodissected Perfused VesselsThomas L. PalloneRenal Microperfusion TechniquesCharles S. Wingo, I. David Weiner, and Shen-Ling XiaHow to Design a Clinical TrialBryan M. Curtis, Brendan J. Barrett, and Patrick S. ParfreyConclusionMichael S. GoligorskyIndex