Description
Humana Stem Cell Protocols 2014 Edition by Ivan N. Rich
This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated cultured in vitro detected and measured for specific applications. These applications range from those in basic stem cell and veterinary research to toxicology cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research. Table of contents : 1. A Short Primer in Stem Cell Biology Ivan N. Rich2. Measurement of Hematopoietic Stem Cell Proliferation Self-Renewal and Expansion PotentialIvan N. Rich3. Measuring the Aging Process in Stem CellsYi Liu Gary Van Zant and Ying Liang4. Measuring the Potency of a Stem Cell TherapeuticHolli Harper and Ivan N. Rich5. Culturing Protocols for Human Multipotent Adult Stem CellsBart Vaes Sara Walbers Kristel Gijbels David Craeye Robert Deans Jef Pinxteren and Wouter van't Hof6. The Isolation of Murine Bone Marrow Scavenging Sinusoidal Endothelial CellsPeter A. M. McCourt Ana Oteiza and Susan K. Nilsson7. Analysis of Circadian Rhythms in Embryonic Stem CellsJiffin K. Paulose Edmund B. Rucker III Vincent M. Cassone8. Measuring Stem Cell Circadian RhythmsWilliam Hrushesky and Ivan N. Rich9. Cryopreservation of Human Pluripotent Stem Cells: A General ProtocolTamamichi Miyazaki and Hirofumi Suemori10. Biological Differences Between Native and Cultured Mesenchymal Stem Cells - Implications for TherapiesElena Jones and Richard Schaefer11. The Use of Multiparameter Flow Cytometry and Cell Sorting to Characterize Native Human Bone Marrow Mesenchymal Stem Cells (MSC)Sally Boxall and Elena Jones12. High Yield Recovery of Equine MEsenchymal Stem Cells from Umbilical Cord Matrix/Wharton's Jelly Using a Semi-Automated ProcessTimo Z. Nazari-Shafti Ivone G. Bruno Rudy Martinez Michael E. Coleman Eckhard U. Alt and Scott R. McClure13. Isolation and Functional Assessment of Cutaneous Stem CellsYanne S. Doucet and David M. Owens14. Isolation of Adult Stem Cell Populations from the Human CorneaMatthew J. Branch Wing-Yan Yu Carl Sheridan and Andrew Hopkinson15. Advanced Imaging and Tissue Engineering of the Human Limbal Epithelial Stem Cell Niche Isobel Massie Marc Dziasko Alvena Kureshi Hannah J. Levis Louise Morgan Michael Neale Radhika Sheth Victoria E. Tovell Amanda J. Vernon James L. Funderburgh and Jule T. Daniels16. Isolation and Characterization of Stem Cells in the Adult Mammalian OvarySeema Parte Hiren Patel Kalpana Sriraman and Deepa Bhartiya17. Clonal Culture of Adult Mouse Lung Epithelial Stem/Progenitor CellsJonathan L. McQualter and Ivan Bertoncello18. Culture and Characterization of Mammary Cancer Stem Cells in MammospeheresEleonora Piscitelli Cinzia Cocola Frank Rudiger Thaden Paride Peluchi Brian Gray Giovanni Bertalot Alberto Albertini Rolland Reinbold and Ileana Zucchi19. Isolation and Culture of Primary Glioblastoma Cells from Human Tumor SpecimensSascha Seidel Boyan K .Garvalov and Till Acker