Description
Springer The Complement System Innovative Diagnostic and Research Protocols 2021 Edition by Lubka T. Roumenina
This Volume Describes A Range Of Methods Used In Complement Laboratories And How To Interpret The Data. Chapters Detail Methods For Depletion Of Igg And Igm, Quantification Of Complement Proteins, C3Dg Quantification, Complement C3 Deposition On Endothelial Cells, Anti-C1Q Auto-Antibodies, And Methods For Assessment Of Interactions Of Proteins With Heme. Written In The Highly Successful Methods In Molecular Biology Series Format, Chapters Include Introductions To Their Respective Topics, Lists Of The Necessary Materials And Reagents, Step-By-Step, Readily Reproducible Laboratory Protocols, And Tips On Troubleshooting And Avoiding Known Pitfalls.Cutting-Edge And Clearly Written, The Complement System: Innovative Diagnostic And Research Protocols Aims To Ensure Successful Results In The Further Study Of This Vital Field. Table Of Contents : - 1. The Benefits Of Complement Measurements For The Clinical Practiceanne Grunenwald And Lubka T. Roumenina2. Method For Depletion Of Igg And Igm From Human Serum As Naive Complement Sourceseline A. Zwarthoff, Simone Magnoni, Piet C. Aerts, Kok P. M. Van Kessel, And Suzan H.M. Rooijakkers3. Quantification Of Complement Proteins With Special Reference To C1Q: Multiplex Vs Elisa Vs Rocket Immunoelectrophoresis Vs Nephelometry Kerstin Sandholm, Barbro Persson, Sliva Abdalla, Camilla Mohlin, Bo Nilsson, And Kristina N Ekdahl4. C3Dg Quantification By Peg-Precipitation And Elisa Or Trifmaanne Troldborg And Jens Christian Jensenius5. Quantification Of Porcine Complement Activation Fragment C3A By A Neoepitope Based Enzyme-Linked Immunosorbent Assayper H. Nilsson, Kristin Pettersen, Martin Oppermann, Espen Skjeflo, Hilde Fure, Dorte Christiansen, And Tom Eirik Mollnes6. Sheep Erythrocytes Preparation For Hemolytic Tests Exploring Complement Functional Activitiesmelchior Chabannes, Pauline Bordereau, Paula Vieira Martins, And Marie-Agnes Dragon-Durey7. Hemolytic Tests Exploring Factor H Functional Activitiesmelchior Chabannes, Shambhuprasad K Togarsimalemath, And Marie-Agnes Dragon-Durey8. Functional Hemolytic Test For Complement Alternative Pathway Convertase Activitymarloes A.H.M. Michels, Nicole C.A.J. Van De Kar, Elena B. Volokhin, And Bert (L) P.W.J. Van Den Heuvel9. Complement C3 Deposition On Endothelial Cells Revealed By Flow Cytometryidris Boudhabhay, Anne Grunenwald, And Lubka T. Roumenina10. Anti-C1Q Auto-Antibodies: Standard Quantification And Innovative Elisa Kinga Csorba, Lucias Schirmbeck, Denise Dubler, And Marten Trendelenburg11. Anti-C1-Inhibitor Auto-Antibodies Detection By Elisa Chiara Suffritti, Sonia Caccia, Silvia Berra, Debora Parolin, And Marco Cicardi12. Anti-Ficolin-2 And Anti-Ficolin-3 Autoantibodies Detection By Elisachantal Dumestre-Perard And Nicole M. Thielens13. Detection Of Anti-C3B Autoantibodies By Elisamaria Radanova, Lubka Roumenina, And Vasil Vasilev14. Detection Of Complement Factor B Autoantibodies By Elisamihaly Jozsi And Barbara Uzonyi15. Detection Of C3 Nephritic Factor By Hemolytic Assaymelchior Chabannes, Veronique Fremeaux-Bacchi, Sophie Chauvet16. Detection Of Genetic Rearrangements Inthe Regulators Of Complement Activation Rca Cluster By High Throughput Sequencing And Mlpajesus Garcia-Fernandez, Susana Vilches-Arroyo, Leticia Olavarrieta, Julian Perez-Perez, And Santiago Rodriguez De Cordoba17. Complement Detection In Mouse Kidneys By Immunofluorescencejennifer Laskowski And Joshua M. Thurman18. Complement Detection In Human Tumors By Immunohistochemistry And Immunofluorescencemarie V. Daugan, Margot Revel, Laetitia Lacroix, Catherine Sautes-Fridman, Wolf H. Fridman, And Lubka T. Roumenina19. Analysis Of The Ligand Recognition Specificities Of Human Ficolins Using Surface Plasmon Resonancenicole M. Thielens, Evelyne Gout, Monique Lacroix, Jean-Baptiste Reiser, And Christine Gaboriaud20. Methods For Assessment Of Interactions Of Proteins With Heme - Application For Complement Proteins And Immunoglobulinsmargot Revel And Jordan D. Dimitrov21. Evaluation Of Binding Kinetics And Thermodynamics Of Antibody-Antigen Interactions And Interactions Involving Complement Proteinssofia Rossini And Jordan D. Dimitrov22. Purification Of Human Complement Component C4 And Sample Preparation For Structural Biology Applicationsalessandra Zarantonello, Sofia Mortensen, Nick S. Laursen, And Gregers R. Andersen